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4. Mihnea Bostina

Associate Professor Mihnea Bostina

Job description:

Research interests:

Structural biology, viral pathogenesis and molecular virology

Current research:

Our research interests are in understanding molecular architectures and relating those structures to their functional mechanisms. To do this, we are employing cryo-electron microscopy and electron tomography combined with image analysis techniques.

Bostina Lab 2023

Alex Crowe, Nick Jadav, James Hodgkinson-Bean, Francesca Hills, Georgia Rutter, Alice Eruera, Mihnea Bostina, Léna Cassin, Rosheny Kumaran, Fangfang Chang, Klem McJarrow-Keller, Laura Burga

Lab group

Dr Laura Burga			Alice Eruera
Research Fellow			Postdoctoral fellow

Postgraduate students

James Hodgkinson-Bean	Georgia Rutter	Francesca Hills
PhD Student	PhD student	PhD student
Nick Jadav	Léna Cassin	Rosheny Kumaran
PhD Student	MSc Student	PhD student
Alex Crowe	Daniel Huang	Klem McJarrow-Keller
PhD Student	MSc Student	MSc student

Research projects

• Seneca Valley Virus

 Seneca Valley Virus (SVV) is a novel oncolytic RNA virus belonging to the Picornaviridae family. SVV has an exclusive tropism for cancer cells and has shown promising results in several clinical trials. Recently, the anthrax toxin receptor, ANTXR1, has been identified as the high-affinity cellular receptor for SVV in multiple cell types. Interestingly, this is the first example of a common shared receptor between a mammalian virus and a bacterial toxin. Our study of SVV follows several directions: i) the structural biology of SVV interaction with receptor and host antibodies; ii) the cellular remodeling induced by SVV infection, and iii) developing SVV for treatment of breast cancer.

• Bacteriophages

Bacteriophages holds an immense potential as tools against antibiotic resistant bacteria, agricultural biocontrol and waste water treatment. This project aims to describe the structures of several bacteriophages.. (Collaboration with Peter Fineran)

• Iridovirus

Iridoviruses are large double-stranded DNA viruses infecting a range of insects.  They have an internal lipid membrane encapsidated into a fiber-covered icosahedral capsid. In special circumstances, the capsids self-assemble into crystal colloidal arrays refracting light in a limited wavelength domain. Understanding the architecture of the capsid in detail can be instrumental in controlling the formation of assembled arrays with desired optical properties important for photonic and material applications. The goal of this project is to obtain a high resolution structure of capsid and understand the role  played by capsid fibers in regulating the array formation. (Collaboration with Vernon Ward)

For details about Diploma, MSc or PhD projects, please contact me at mihnea.bostina@otago.ac.nz

Lab alumni

Fangfang Chang (2023) • Sai Velamoor (2017-2023) • Shakeel Waqqar (2017-2022) • Isa de Vries (2022) •  En Watanabe (2020-2022) •  Daniel Huang (2021) •  An Pham (2021) •  Harry McFarlane (2020) •  Milly Oldfield (2020) •  Nadishka Jayawardena (2016-2020) •  Sean Mackay (2019) •  Kai Lee (2018-2019) • Cormac McCarthy (2016-2019) •  Guillaume Ems (2019) •  Alice Dunn (2016-2018) •  WonMo Kim (2018) •  Ana Rivera (2018) • Sally Kim (2014-2018) •  Brian Tong (2015-2017) •  William Szabo (2016) •  Ryan Murphy-Dawes (2015) •  Chelsea Harris (2015) •  Mike Strauss (2014) •  Mariya Yevstigneyeva (2014) •  Danni Chen (2014)

Publications:

Recent publications

• McSweeney, AM, AR Eruera, GM McKenzie-Goldsmith, JC Bouwer, SHJ Brown, LA Stubbing, JG Hubert, R Shrestha, KJ Sparrow, MA Brimble, LD Harris, GB Evans, M Bostina, KL Krause, VK Ward. 2024. Activity and cryo-EM structure of the polymerase domain of the human norovirus ProPol precursor. Journal of Virology. 184391
• Eruera, A-R, J Hodgkinson-Bean, GL Rutter, FR Hills, R Kumaran, AJM Crowe, N Jadav, F Chang, K McJarrow-Keller, F Jorge, J Hyun, H Kim, B Ryu, M Bostina^*. 2024. Ejectosome of Pectobacterium bacteriophage ΦM1. PNAS Nexus, 3(9):pgae416.
• Mackay, SM, B Sutherland, RA Easingwood, A Hopkins, M Bostina, Eng Wui Tan. 2024. Evidence for phospholipid self-organisation in concentrated ammonia-water environments. BBA Biomembranes. 1866(8):184391.
• Hills, FR, AR Eruera, J  Hodgkinson-Bean, F Jorge, R Easingwood, SHJ Brown, JC Bouwer, Li YP, LN Burga, M Bostina. 2024. Variation in structural motifs within SARS-related coronavirus spike proteins. PLoS Pathogens, e1012158.
• Jadav N, Velamoor S, Huang D, Cassin L, Hazelton N, Eruera AR, Burga LN, M Bostina*. 2023. Beyond the surface: Investigation of tumorsphere morphology using volume electron microscopy. J Struct Biol.;215(4):108035.
• Wierenga, JR, RM Grimwood, HS Taylor, S Hunter, LS Argilla, T Webster, L Lim, R French, H Schultz, F Jorge, M Bostina, L Burga, P Swindells-Wallace, EC Holmes, K McInnes, KJ Morgan, JL Geoghegan. 2023. Total infectome investigation of diphtheritic stomatitis in yellow-eyed penguins (Megadyptes antipodes) reveals a novel and abundant megrivirus. Vet Microbiol, 109895.
• Waqqar S, K Lee, B Lawley, T Bilton, ME Quinones-Mateu, M Bostina*, LN Burga*. 2023. Directed Evolution of Seneca Valley Virus in Tumorsphere and Monolayer Cell Cultures of a Small-Cell Lung Cancer Model. Cancers 15(9), 2541
• Wierenga JR, Morgan KJ, Hunter S, Taylor HS, Argilla LS, Webster T, Dubrulle J, Jorge F, Bostina M, Burga L, Holmes EC, McInnes K, Geoghegan JL. 2023. A novel gyrovirus is abundant in yellow-eyed penguin (Megadyptes antipodes) chicks with a fatal respiratory disease. Virology, 579: 75–83
• Peng Y, Liu Y, Hu Y, Chang F, Wu Q, Yang J, Chen J, Teng S, Zhang J, He R, Wei Y, Bostina M, Luo T, Liu W, Qu X, Li Y-P. 2022. Monoclonal antibodies constructed from COVID-19 convalescent memory B cells exhibit potent binding activity to MERS-CoV spike S2 subunit and other human coronaviruses. Frontiers in Immunology 13.
• Jayawardena N, McCarthy C, Wang I, Waqqar S, Burga LN, Strauss M*, Bostina M*. 2022. Characterisation of a Seneca Valley virus thermostable mutant. Virology. 575:74-82.
• Warring, SL, LM Malone, J Jayaraman, RA Easingwood, L Rigano, RA Frampton, SB Visnovsky, SM Addison, L Hernandez, AR Pitman, E Lopez Acedo, T Kleffmann, MD Templeton, M Bostina, PC Fineran. 2022. A lipopolysaccharide-dependent phage infects a pseudomonad phytopathogen and can evolve to evade phage resistance. Environ Microbiol. doi.org/10.1101/2022.06.10.495718
• Wang Y, SK Gupta, G Ems, N Jayawardena, M Strauss*, M Bostina*. 2022. Cryo-EM structure of a possum enterovirus. Viruses.14(2), 318.
• Harfoot R, B Lawley, LC Hernández, J Kuang, J Grant , JM Treece, S Lequeux, R Day, S Jack, JAL. Stanton, M Bostina, JE Ussher, ME. Quiñones-Mateu. 2022. Characterization of the First SARS-CoV-2 Isolates from Aotearoa New Zealand as Part of a Rapid Response to the COVID-19 Pandemic. Viruses.14(2), 366.
• Jayawardena N, Miles LA, Burga LN, Rudin C, Wolf M, Poirier JT, Bostina M. 2021. N-Linked Glycosylation on Anthrax Toxin Receptor 1 Is Essential for Seneca Valley Virus Infection. Viruses, 13(5):769.
• Velamoor S, A Mitchell, M Bostina, D Harland. 2021. Processing hair follicles for transmission electron microscopy. Exp Dermatol. 31: 110-121.
• Burga, LN and M Bostina. 2021. Oncolytic Viruses. Chapter in Encyclopedia of Virology, 4th edition. 658-671. Elsevier. Editors in Chief: D, Bamford and M. Zuckerman
• Velamoor, S, S Lequeux, RA Easingwood, A Mitchell, J Plowman, M Bostina, DP Harland. 2021. A Correlative Fluorescent and Electron Microscopic Technique for Ultralocalization of Trichocyte Keratins. Chapter in Applications of Microscopy in Materials and Life Sciences, vol 11. 243-250. Springer, Singapore.
• Humbel, B M, S Velamoor, A Mitchell, and M Bostina. 2021. Volume Microscopy of Nudivirus Infected Cells. Chapter in Applications of Microscopy in Materials and Life Sciences, vol 11. 251-259. Springer, Singapore.
• Velamoor S, A Mitchell, BM Humbel, W Kim, C Pushparajan, G Visnovksy, LN Burga, M Bostina^*. 2020. Visualizing Nudivirus Assembly and Egress. mBio, 11 (4):e-1333-20.
• Jayawardena N, JT Poirier, LN Burga^*, M Bostina^*. 2020. Virus-receptor interactions and virus neutralization: insights for oncolytic virus development. Oncolytic Virotherapy. 9:1-15.
• Velamoor S, M Richena, A Mitchell, S Lequeux, M Bostina, D Harland. 2020. High-pressure freezing followed by freeze substitution of a complex and variable density miniorgan: the wool follicle. J Microsc. 278(1):18-28. 
• Jayawardena N, LN Burga, JT Poirier, M Bostina. 2019. Virus–Receptor Interactions: Structural Insights For Oncolytic Virus Development. OncolyticVirotherapy Volume 8:39-56.
• Kim SH, A Mercer, A Mitchell,  JR de Miranda, V Ward, F Mondet, M Bostina. 2019. Viral infections alter antennal epithelium ultrastructure in honey bees. J Invertebr Pathol 168:107252.
• McCarthy C, N Jayawardena, LN Burga^*, M Bostina^*. 2019. Developing Picornaviruses for Cancer Therapy. Cancers (Basel). 11(5). pii: E685.
• Bostina, M. 2019. Monoclonal antibodies point to Achilles’ heel in picornavirus capsid. PLOS Biology. doi:10.1371/journal.pbio.3000232
• Harland, DP, V Novotna, M Richena, S Velamoor, M Bostina, AJ McKinnon. 2019. Helical twist direction in the macrofibrils of keratin fibres is left handed. J Struct Biol. https://doi.org/10.1016/j.jsb.2019.04.007
• Watson, BNJ, RA Easingwood, B. Tong, M. Wolf, GPC Salmond, RHJ Staals, M Bostina^*, PC Fineran^*. 2019. Different genetic and morphological outcomes for phages targeted by single or multiple CRISPR-Cas spacers. Phil. Trans. R. Soc. B. 374(1772):20180090 
• Jayawardena, N, LN Burga, RA Easingwood, Y Takizawa, M Wolf*, M Bostina*. 2018. Structural basis for Anthrax Toxin Receptor 1 recognition by Seneca Valley Virus. PNAS. 13;115(46):E10934-E10940.
• Strauss, M, N Jayawardena, E Sun, RA Easingwood, LN Burga*, M Bostina*. 2017. Cryo-EM Structure of Seneca Valley Virus Procapsid. Journal of Virology 92(6):e01927-17.
• Miles L, LNBurga, E Gardner, MBostina, JT Poirier, C Rudin. 2017. Anthrax toxin receptor 1 (ANTXR1) is the cellular receptor for Seneca Valley Virus in neuroendocrine cancers. Journal of Clinical Investigation. 127(8):2957-2967.
• Fagerlund, RD, ME Wilkinson, O Klykov, A Barendregt, FG Pearce, SN Kieper, HWR Maxwell, A Capolupo, AJR Heck, KL Krause, M Bostina, RA Scheltema, RHJ Staals, PC Fineran. 2017. Spacer capture and integration by a type I-F Cas1:Cas2-3 CRISPR adaptation complex. PNAS. 14(26):E5122-E5128.

     A complete list of publication on pubmed.