Molecular Virology, Viral Pathogenesis and Epidemiology
I am a virologist and study the molecular nature of virus-host interactions. The goal of this research is to create knowledge base to identify novel drug targets and design host-directed antiviral therapies.
PhD and MSc research projects are available in lab; get in touch if you are motivated to do contemporary research in the field of molecular virology. General information on PhD/MSc admissions and scholarships is available on University of Otago Graduate Research School website.
The current focus of research in my lab is influenza virus. Influenza virus continues to cause regular seasonal epidemics, intermittent unpredictable pandemics and deadly zoonotic outbreaks. These events have huge impact on global public and animal health as well as economy and productivity. The lack of a universal influenza vaccine, variable efficacy of annually-formulated influenza vaccines and rapid emergence of drug-resistant influenza variants press the need to develop effective and long-lasting anti-influenza strategies. To contribute to this over-arching goal, my research focuses on identifying the host factors, which are critically involved in influenza virus infection. To this end, my research group have discovered that multiple members of a family of host proteins known as histone deacetylases (HDACs) possess the inherent ability to inhibit influenza virus infection. However, influenza virus antagonises those HDACs by various mechanisms to undermine their antiviral function. We continue to study the molecular mechanisms of such influenza virus-HDACs interplay. Furthermore, we are also studying the role of histone acetyltransferases (HATs), the counterparts of HDACs, in influenza virus infection. The aim of current research is to elucidate the role of host acetylation machinery in influenza virus infection and determine its potential for host-directed anti-influenza therapy.
Henry D Galvin and Matloob Husain*. Influenza A virus-induced host caspase and viral PA-X antagonise the antiviral host factor, histone deacetylase 4. Journal of Biological Chemistry, DOI: 10.1074/jbc.RA119.010650 (2019).
Rajesh Lamichhane, Henry Galvin, Rachel F Hannaway, Sara M de la Harpe, Fran Munro, Joel DA Tyndall, Andrea J Vernall, John L McCall, Matloob Husain, and James E Ussher. Type I interferons are important co‐stimulatory signals during T cell receptor mediated human MAIT cell activation. European Journal of Immunology DOI: 10.1002/eji.201948279 (2019).
Ashley N Nutsford, Henry D Galvin, Farjana Ahmed, and Matloob Husain*. The Class IV human deacetylase, HDAC11, exhibits anti‐influenza A virus properties via its involvement in host innate antiviral response. Cellular Microbiology, e12989 (2018). Article Link
Prashanth T Nagesh, Mazhar Hussain, Henry D Galvin, and Matloob Husain*. Histone deacetylase 2 is a component of influenza A virus-induced host antiviral response. Frontiers in Microbiology, 8: 1315 (2017). Article Link
Mazhar Hussain, Henry D Galvin, Tatt Y Haw, Ashley N Nutsford, and Matloob Husain*. Drug resistance in influenza A virus: the epidemiology and management. Infection and Drug Resistance 10: 121-134 (2017). Article Link
Subrata Barman, Petr S Krylov, Jasmine C Turner, John Franks, Robert G Webster, Matloob Husain, Richard J Webby. Manipulation of neuraminidase packaging signals and hemagglutinin residues improves the growth of A/Anhui/1/2013 (H7N9) influenza vaccine virus yield in eggs. Vaccine 35 (10): 1424-1430 (2017). Article Link
Matloob Husain*, Andrew Gray, and Richard Carlos Torr. The awareness and uptake of free flu vaccination offered to University of Otago staff. NZ Medical Journal 129 (1443): 92-93 (2016). Article Link
Da-Yuan Chen and Matloob Husain*. Caspase-mediated degradation of host cortactin that promotes influenza A virus infection in epithelial cells. Virology 497: 146-156 (2016). Article Link
Prashanth T Nagesh and Matloob Husain*. Influenza A virus dysregulates host histone deacetylase 1 that inhibits viral infection in lung epithelial cells. Journal of Virology 90 (9): 4614-4625 (2016). Article Link
Vivek Poonthiyil, Prashanth T Nagesh, Matloob Husain, Vladimir B Golovko, and Antony J Fairbanks. Gold nanoparticles decorated with sialic acid terminated bi-antennary N-glycans for the detection of influenza virus at nanomolar concentrations. ChemistryOpen 4 : 708-716 (2015). Article Link *Published with cover page illustration and article highlight.
Hassan Zaraket, Tatiana Baranovich, BS Kaplan, R Carter, MS Song, JC Paulson, JE Rehg, J Bahl, JC Crumpton, J Seiler, M Edmonson, G Wu, E Karlsson, T Fabrizio, H Zhu, Y Guan, Matloob Husain, S Schultz-Cherry, S Krauss, R McBride, RG Webster, EA Govorkova, J Zhang, CJ Russell, and Richard J Webby. Mammalian adaptation of influenza A (H7N9) virus is limited by a narrow genetic bottleneck. Nature Communications 6 : 6553 (2015). Article Link
Matloob Husain*. Avian influenza A (H7N9) virus infection in humans: Epidemiology, Evolution, and Pathogenesis. Infection, Genetics and Evolution. 28 : 304-312 (2014). Article Link
Matloob Husain* and CY Cheung. Histone deacetylase 6 inhibits influenza A virus release by downregulating the trafficking of viral components to the plasma membrane via its substrate acetylated microtubules. Journal of Virology 88 (19) : 11229-11239 (2014). Article Link
Singh, S.P., Gundavarapu, S., Smith, K.R., Chand, H.S., Saeed, A.I., Mishra, N.C., Hutt, J., Barrett, E.G., Husain, M., Harrod, K.S., Langley, R.J., Sopori, M.L. Gestational Exposure of Mice to Secondhand Cigarette Smoke Causes Bronchopulmonary Dysplasia Blocked by the Nicotinic Receptor Antagonist Mecamylamine. Environmental Health Perspectives 121: 957-964 (2013).
Husain M* and Harrod KS. Enhanced acetylation of alpha-tubulin in influenza A virus infected epithelial cells. FEBS Letters 585 (1): 128-132 (2011). Article Link
Husain M* and Harrod KS. Influenza A virus-induced caspase-3 cleaves the histone deacetylase 6 in infected epithelial cells. FEBS Letters 583 (15): 2517-2520 (2009). Article Link
Husain, M., A. Weisberg and B. Moss (2007). Resistance of a vaccinia virus A34R-deletion mutant to spontaneous rupture of the outer membrane of progeny virions on the surface of infected cells. Virology 366 (2): 424-432.
Husain, M., A. Weisberg and B. Moss (2007). Sequence-independent targeting of trans-membrane proteins synthesized within vaccinia virus factories to nascent viral membranes. Journal of Virology 81 (6): 2646-2655. *Article selected for journal spotlight.
Husain, M., A. Weisberg and B. Moss (2006). Existence of an operative pathway from the endoplasmic reticulum to the immature poxvirus membrane. Proceedings of National Academy of Science USA 103 (51): 19506-19511.
Husain, M. and B. Moss (2005). Role of receptor-mediated endocytosis in the formation of vaccinia virus extracellular enveloped particles. Journal of Virology 79 (7): 4080-4089.
Husain, M. and B. Moss (2003). Evidence against an essential role of COPII-mediated cargo transport to the endoplasmic reticulum-Golgi intermediate compartment in the formation of the primary membrane of vaccinia virus. Journal of Virology 77 (21): 11754-11766.
Husain, M. and B. Moss (2003). Intracellular trafficking of a palmitoylated membrane-associated protein component of enveloped vaccinia virus. Journal of Virology 77 (16): 9008-9019.
Husain, M., A. Weisberg and B. Moss (2003). Topology of epitope-tagged F13 protein, a major membrane component of extracellular vaccinia virions. Virology 308 (2): 233-242. *Published with cover page illustration.
Husain, M. and B. Moss (2002). Similarities in the induction of post-Golgi vesicles by the vaccinia virus F13 protein and phospholipase D. Journal of Virology 76 (15): 7777-7789.
Husain, M. and B. Moss (2001). Vaccinia virus F13 protein with a conserved phospholipase catalytic motif induces colocalization of the B5 envelope glycoprotein in post-Golgi vesicles. Journal of Virology 75 (16): 7528-7542.
Husain, M. and C. M. Gupta (1997). Interactions of viral matrix protein and nucleoprotein with the host cell cytoskeletal actin in influenza viral infection. Current Science 73:40-47.
(Past and Present)
Health Research Council
HS and JC Anderson Trust
Lottery Health Board
School of Biomedical Sciences
University of Otago
Flu targets. He Kitenga 150, 2019 ed. pp 37-39