Second Semester - 18 points
Microbial technologies, including production and purification of recombinant proteins in microbes, immune technologies, bioremediation and microbial biodegradation networks. Commercialisation of biotechnology products.
Most microbes are not pathogens, rather their diversity has been exploited for a range of beneficial purposes, with much of modern biotechnology studying and exploiting the ability of microbes to grow rapidly and to high levels in fermenters, to be manipulated genetically for the production of recombinant proteins, to be used in the environment for practices such as bioremediation and as technological stepping stones in discovery research.
This course in Microbial Biotechnology focuses upon the growth, manipulation and use of microbes for beneficial purposes. In particular you will:
This course is for students interested in the use of molecular microbiology for the production of beneficial products and applications. It ranges from large-scale fermentation to recombinant protein expression and purification, to concepts underlying the commercialisation of biotechnology.
The MICR 336 lecture series typically includes lectures on:
The MICR 336 lab classes will expose you to the use of molecular biology in biotechnology. In particular, during the 4-week lab course you will set up a gene for protein expression, express the protein in Escherichia coli and then purify the protein using a recombinant peptide tag built into the construct during cloning. You will also design your own process to isolate, express and purify a recombinant protein.
Mondays and Wednesdays 1-1.50pm
Weeks 32-34, 36, Tuesdays 2-6pm, all day Wednesdays
There is no required text for this course but you will be directed to relevant scientific papers during lectures and you may like to read Molecular Biotechnology: Principles and applications of recombinant DNA by B.R. Glick and J.J. Pasternak, 2003 (3rd Edition), ASM Press, which is held in the Science Library.
Students are encouraged to contact staff by email to make arrangements for a time to discuss course-related matters.
For more information on this course, please contact Dr Sergio Morales.