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Te Tari Moromoroiti me te Ārai Mate

Sesquicentennial Distinguished Professor Greg Cook


Research interests:

Microbial physiology and biochemistry, antimicrobial resistance, drug discovery, molecular microbiology, structural biology

Current research:

Research projects in the Cook laboratory are multidisciplinary, spanning human biomedical and agritech/biotech, and covers basic to translational research. We interact with multiple end users in academia, government, industry and pharma.  Research from the Cook laboratory is aimed at providing fundamental knowledge on the metabolism and energetics of bacterial pathogens like Mycobacterium tuberculosis and multidrug-resistant Gram-negative pathogens. A major goal of this research is to translate these findings into new drug target development to combat antimicrobial-resistance (AMR) and tolerance, and provide molecular and mechanistic insight into the mode of action of antimicrobials (old and new).  A second major theme of the Cook laboratory is aimed at preserving human, animal and plant health in the face of AMR through the discovery and implementation of compounds that are used exclusively in animals and plants to combat environmental pathogens like mastitis-causing bacteria and fungal pathogens of plants. The goal of this work is to break the link between human and agricultural use of the same classes of antimicrobials and safeguard against the potential for antimicrobial resistance determinant transfer via the food chain.   Part of this theme includes discovering new inhibitors for management of greenhouse gas emissions in ruminant animals and soils.

 Appointments and Honours:

  • Director, The Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland. http://www.mauricewilkinscentre.org/about-us/our-people.aspx
  • Chair-elect, Gordon Research Conference on Bioenergetics (vice Chair 2019, Chair 2021).
  • University of Otago Research Group Award 2017,Otago Bacterial Energetics and Antimicrobial Resistance Group
  • University of Otago Distinguished Research Medal, 2014 (University of Otago's highest distinction)
  • Fellow of the Royal Society New Zealand Te Apārangi, 2013
  • James Cook Fellow, 2012-2014
  • 8th Sir William Dunn Fellow, 2009 (Cambridge U.K.)

Research projects 

  • A. Bacterial energetics as a new target space for drug development

  • B. Fueling persistence: adaptation of Mycobacterium tuberculosis to hypoxia and low energy conditions

  • C. Antimicrobial resistance and finding new drugs for environmental microorganisms

These projects utilize modern technologies (e.g. proteomics, transcriptomics, molecular biology and structural biology) to address important questions in bacterial adaptation to environment.


Greg Cook

Cook Lab Jan 2020

Current group members

Cook19 colour

Sesquicentennial Distinguished Professor Gregory M. Cook | MSc(Hons), DPhil, FRSNZ
Microbiology and Immunology
University of Otago
Tel/+64 03 479 7722 | Mobile/+64 21 244 7257

Otago Bacterial Energetics and Antimicrobial Resistance (OBEAR) Group

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James Cheung - Assistant Research Fellow

Dr Rachel Darnell - Postdoctoral Fellow Dr Scott Ferguson - Scientific Officer Dr Kiel Hards - Postdoctoral Fellow

 

Liam

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Dr Liam Harold - Assistant Research Fellow

Alan Hughes - Senior Research Technician    Alex Krause - Research Assistant Dr Scott Lawrence - Postdoctoral Fellow 
NicolaM Mathew McNeil

 

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 Nicola Marechal - Lab Manager  Dr Matthew McNeil - Research Fellow  Sonya Mros - Senior Research Technician   

 

Postgraduate students

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Cara Adolph - PhD Student

Peter Christensen - PhD Student

Carmel Donald - PhD Student

Nichaela Harbison-Price - PhD Student 

 

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Francesca Todd Rose - PhD Student Euan Russell - PhD Student Essie Van Zuylen - PhD Student  

 Heath Ryburn

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 Alaska

 
Heath Ryburn - MSc Student    Natalie Waller - MSc Student Alaska Kotan - MSc Student  
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Kathryn Waller - BSc (Hons)  Chloe Estoque - BSc (Hons) Hannah Hodgkinson - BSc (Hons) GENE Laura Keighley - BSc (Hons)
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Recent publications 

View Google Scholar profile

2020

Tong, J.T.W., Kavianinia, I., Fergusson, S.A., Cook, G.M., Harris, P.W.R. and M.A. Brimble. Synthesis of paenipeptin Cʹ analogues employing solution-phase CLipPA chemistry. Organic & Biomolecular Chemistry 18:4381-4385 (2020)

Yim, V., Kavianinia, I., Knottenbelt, M.K., Ferguson, S.A.,Cook, G.M., Anand, V., Swift, S., Harris, P.W.R. and M.A. Brimble. “CLipP”ing on lipids to generate antibacterial lipopeptides. Chemical Science 11:5759–5765 (2020)

Grinter, R., Ney, B., Brammananth, R.,Barlow, C.K., Cordero, P.R.F., Gillett, D.L., Izoré, T., Cryle,M.J., Harold, L.K., Cook, G.M., Taiaroa, G., Williamson,D.A., Warden, A.C., Oakeshott, J.G., Taylor, M.C.,Crellin, P.K., Jackson, C.J., Schittenhelm, R.B., Coppel,R.L. and C. Greening. Cellular and structural basis of synthesis of the unique intermediatedehydro-F420-0 in mycobacteria. mSystems 5:e00389-20.

McNeil, M.B., Ryburn, H., Harold, L., Tirados, J. and G.M. Cook. Transcriptional inhibition of the F1F0-type ATP synthase has bactericidal consequences on the viability of mycobacteria. Antimicrobial Agents and Chemotherapy (2020) May 18:AAC.00492-20. doi: 10.1128/AAC.00492-20. Online ahead of print.

Santoso, K.T., Brett, M.W., Cheung, C.-Y., Cook, G.M., Stocker, B.L. and M.S.M. Timmer.  Synthesis of functionalised chromonyl-pyrimidines and their potential as antimycobacterial agents. ChemistrySelect 5:4347 –4355 (2020)

Rineh A, Soren O, McEwan T, Ravikumar V, Poh WH, Azamifar F, Naimi-Jamal MR, Cheung CY, Elliott AG, Zuegg J, Blaskovich MAT, Cooper MA, Dolange V, Christodoulides M, Cook GM, Rice SA, Faust SN, Webb JS, and MJ Kelso. Discovery of cephalosporin-3 -diazeniumdiolates that show dual antibacterial and antibiofilm effects against Pseudomonas aeruginosa clinical cystic fibrosis isolates and efficacy in a murine respiratory infection model. ACS Infectious Diseases doi:10.1021/acsinfecdis.0c00070 (2020).

Harbison-Price N, Ferguson SA, Heikal A, Taiaroa G, Hards K, Nakatani Y, Rennison D, Brimble MA, El-Deeb IM, Bohlmann L, McDevitt CA, von Itzstein M, Walker MJ, Cook GM.  Multiple Bactericidal Mechanisms of the Zinc Ionophore PBT2. mSphere 5 (2020)

Chong SMS, Manimekalai MSS, Sarathy JP, Williams ZC, Harold LK, Cook GM, Dick T, Pethe K, Bates RW, Gruber G.  Antituberculosis Activity of the Antimalaria Cytochrome bcc Oxidase Inhibitor SCR0911. ACS Infect Dis 6:725-737 (2020)

Hards K, Adolph C, Harold LK, McNeil MB, Cheung CY, Jinich A, Rhee KY, Cook GM.  2020. Two for the price of one: Attacking the energetic-metabolic hub of mycobacteria to produce new chemotherapeutic agents. Prog Biophys Mol Biol 152:35-44.

Lee BM, Harold LK, Almeida DV, Afriat-Jurnou L, Aung HL, Forde BM, Hards K, Pidot SJ, Ahmed FH, Mohamed AE, Taylor MC, West NP, Stinear TP, Greening C, Beatson SA, Nuermberger EL, Cook GM, Jackson CJ.  2020. Predicting nitroimidazole antibiotic resistance mutations in Mycobacterium tuberculosis with protein engineering. PLoS Pathog 16:e1008287.

McGowan JE, Harper AD, Davison EK, Jeong JY, Mros S, Harbison-Price N, Van Zuylen EM, Knottenbelt MK, Heikal A, Ferguson SA, McConnell MA, Cook GM, Krittaphol W, Walker GF, Brimble MA, Rennison D.  2020. Substituted sulfonamide bioisosteres of 8-hydroxyquinoline as zinc-dependent antibacterial compounds. Bioorg Med Chem Lett doi:10.1016/j.bmcl.2020.127110:127110.

Nakatani Y, Shimaki Y, Dutta D, Muench SP, Ireton K, Cook GM, Jeuken LJC.  2020. Unprecedented Properties of Phenothiazines Unraveled by a NDH-2 Bioelectrochemical Assay Platform. J Am Chem Soc 142:1311-1320.

2019

Aung HL, Devine TJ, Mulholland CV, Arcus VL, Cook GM.  2019. Tackling tuberculosis in the indigenous people of New Zealand. Lancet Public Health 4:e496.

Buckley BJ, Majed H, Aboelela A, Minaei E, Jiang L, Fildes K, Cheung CY, Johnson D, Bachovchin D, Cook GM, Huang M, Ranson M, Kelso MJ.  2019. 6-Substituted amiloride derivatives as inhibitors of the urokinase-type plasminogen activator for use in metastatic disease. Bioorg Med Chem Lett 29:126753.

Cordero PRF, Grinter R, Hards K, Cryle MJ, Warr CG, Cook GM, Greening C.  2019. Two uptake hydrogenases differentially interact with the aerobic respiratory chain during mycobacterial growth and persistence. J Biol Chem 294:18980-18991.

Darnell RL, Knottenbelt MK, Todd Rose FO, Monk IR, Stinear TP, Cook GM.  2019. Genomewide Profiling of the Enterococcus faecalis Transcriptional Response to Teixobactin Reveals CroRS as an Essential Regulator of Antimicrobial Tolerance. mSphere 4.

Darnell RL, Nakatani Y, Knottenbelt MK, Gebhard S, Cook GM.  2019. Functional characterization of BcrR: a one-component transmembrane signal transduction system for bacitracin resistance. Microbiology 165:475-487.

Ferguson SA, Menorca A, Van Zuylen EM, Cheung CY, McConnell MA, Rennison D, Brimble MA, Bodle K, McDougall S, Cook GM, Heikal A.  2019. Microtiter Screening Reveals Oxygen-Dependent Antimicrobial Activity of Natural Products Against Mastitis-Causing Bacteria. Front Microbiol 10:1995.

Greening C, Geier R, Wang C, Woods LC, Morales SE, McDonald MJ, Rushton-Green R, Morgan XC, Koike S, Leahy SC, Kelly WJ, Cann I, Attwood GT, Cook GM, Mackie RI.  2019. Diverse hydrogen production and consumption pathways influence methane production in ruminants. ISME J 13:2617-2632.

Hards K, Rodriguez SM, Cairns C, Cook GM.  2019. Alternate quinone coupling in a new class of succinate dehydrogenase may potentiate mycobacterial respiratory control. FEBS Lett 593:475-486.

Harold LK, Antoney J, Ahmed FH, Hards K, Carr PD, Rapson T, Greening C, Jackson CJ, Cook GM.  2019. FAD-sequestering proteins protect mycobacteria against hypoxic and oxidative stress. J Biol Chem 294:2903-2912.

Hasenoehrl EJ, Rae Sajorda D, Berney-Meyer L, Johnson S, Tufariello JM, Fuhrer T, Cook GM, Jacobs WR, Jr., Berney M.  2019. Derailing the aspartate pathway of Mycobacterium tuberculosis to eradicate persistent infection. Nat Commun 10:4215.

Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts A, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee K, Ding K, Zhang T, Cook GM.  2019. Pyrazolo[1,5- a]pyridine Inhibitor of the Respiratory Cytochrome bcc Complex for the Treatment of Drug-Resistant Tuberculosis. ACS Infect Dis 5:239-249.

McNeil MB, Cook GM.  2019. Utilization of CRISPR Interference To Validate MmpL3 as a Drug Target in Mycobacterium tuberculosis. Antimicrob Agents Chemother 63.

Momin MAM, Rangnekar B, Sinha S, Cheung CY, Cook GM, Das SC.  2019. Inhalable Dry Powder of Bedaquiline for Pulmonary Tuberculosis: In Vitro Physicochemical Characterization, Antimicrobial Activity and Safety Studies. Pharmaceutics 11.

Mulholland CV, Shockey AC, Aung HL, Cursons RT, O'Toole RF, Gautam SS, Brites D, Gagneux S, Roberts SA, Karalus N, Cook GM, Pepperell CS, Arcus VL.  2019. Dispersal of Mycobacterium tuberculosis Driven by Historical European Trade in the South Pacific. Front Microbiol 10:2778.

Petri J, Nakatani Y, Montgomery MG, Ferguson SA, Aragao D, Leslie AGW, Heikal A, Walker JE, Cook GM.  2019. Structure of F1-ATPase from the obligate anaerobe Fusobacterium nucleatum. Open Biol 9:190066.

Rushton-Green R, Darnell RL, Taiaroa G, Carter GP, Cook GM, Morgan XC.  2019. Agricultural Origins of a Highly Persistent Lineage of Vancomycin-Resistant Enterococcus faecalis in New Zealand. Appl Environ Microbiol 85.

Santoso KT, Cheung CY, Hards K, Cook GM, Stocker BL, Timmer MSM.  2019. Synthesis and Investigation of Phthalazinones as Antitubercular Agents. Chem Asian J 14:1278-1285.

Santoso KT, Menorca A, Cheung CY, Cook GM, Stocker BL, Timmer MSM.  2019. The synthesis and evaluation of quinolinequinones as anti-mycobacterial agents. Bioorg Med Chem 27:3532-3545.

Saw WG, Wu ML, Ragunathan P, Biukovic G, Lau AM, Shin J, Harikishore A, Cheung CY, Hards K, Sarathy JP, Bates RW, Cook GM, Dick T, Gruber G.  2019. Disrupting coupling within mycobacterial F-ATP synthases subunit epsilon causes dysregulated energy production and cell wall biosynthesis. Sci Rep 9:16759.

Zhang AT, Montgomery MG, Leslie AGW, Cook GM, Walker JE.  2019. The structure of the catalytic domain of the ATP synthase from Mycobacterium smegmatis is a target for developing antitubercular drugs. Proc Natl Acad Sci U S A 116:4206-4211.

2018

Bohlmann, L., De Oliveira, D.M.P., El-Deeb, I.M., Brazel, E.B., Harbison-Price, N., Ong, C.-L.Y., Rivera-Hernandez, T., Ferguson, S.A., Cork, A.J., Phan, M.-D., Soderholm, A.T., Davies, M.R., Nimmo, G.R., Dougan, G., Schembri, M.A., Cook, G.M., McEwan, A.G., von Itzstein, M., McDevitt, C.A. and M. J. Walker. Chemical synergy between ionophore PBT2 and zinc reverses antibiotic resistance. mBio 9:e02391-18 (2018)

Buckley, B.J., Aboelela, A., Minaei, E., Jiang, L., Xu, Z., Ali, U., Fildes, K., Cheung, C.Y., Cook, S.M., Johnson, D., Bachovchin, D.A., Cook, G.M., Apte, M., Huang, M., Ranson, M. and M.J. Kelso. 6-Substituted hexamethylene amiloride (HMA) derivatives as potent and selective inhibitors of the human urokinase plasminogen activator for use in cancer. Journal of Medicinal Chemistry 61:8299−8320 (2018) (PMID:30130401)

Hards, K., McMillan, D.G.G., Schurig-Briccio, L.A., Gennis, R.B., Lill, H., Bald, B.D. and G.M. Cook.  Ionophoric effects of the antitubercular drug bedaquiline. Proceedings of the National Academy of Sciences of the United States of America 115:7326-7331 (2018)

Htun, K.S., Fong, Y., Kyaw, A.A., Aung, S.T., Oo, K.Z., Zaw, T., Lockhart, P.J., Russell, B., Cook, G.M., Aung, H.L. and T.M. Hlaing. Microbiome dataset from the upper respiratory tract of patients living with HIV,HIV/TB and TB from Myanmar. Data in Brief 21: 354–357 (2018)

Aung, A.L. Nyunt, W.W., Fong, Y., Russell, B., Cook, G.M. and S.T. Aung. Acquired resistance to antituberculosis drugs. Emerging Infectious Diseases 24, No.11, November (2018)

Mulholland, C.V., Thorpe, D., Cursons, R., Karalus, N., Fong, Y., Arcus, V.L., Cook, G.M. and H.L. Aung. Evaluation of the rapid molecular diagnostic test for the New Zealand Mycobacterium tuberculosis Rangipo strain in a clinical setting. New Zealand Medical Journal 131:70-72 (2018)

Heikal, A., Nakatani, Y., Jiao, W., Wilson, C., Rennison, D., Weimar, M.R., Parker, E.J., Brimble, M.A. and G.M. Cook. Tethering fragment-based drug discovery to identify inhibitors of the essential respiratory membrane protein type II NADH dehydrogenase. Bioorganic & Medicinal Chemistry Letters 28:2239-2243 (2018)

Petri, J.,Shimaki, Y., Jiao, W., Bridges, H.R., Russell, E.R., Parker, E.J., Aragão, D., Cook, G.M. and Y. Nakatani. Structure of the NDH-2 - HQNO inhibited complex provides molecular insight into quinone-binding site inhibitors. BBA Bioenergetics 1859:482-490 (2018)

Taiaroa, G., Harbison-Price, N., Ferguson, S.F., Carter, G.P, Williamson, D.A, Macknight, R.C, Cook, G.M. and A. Heikal. Complete genome sequence of a New Zealand isolate of the bovine pathogen Streptococcus uberis. Genome Announcements 6(9) e00119-18 (2018)

Zhang, S., De Leon Rodriguez, Leung, I.K.H., Cook, G.M., Harris, P.W.R. and M.A. Brimble. Total synthesis and conformational study of the anti-tubercular cyclic peptide callyaerin A bearing a rare rigidifying (Z)-2,3-diaminoacrylamide moiety. Angewandte Chemie International Edition 57:3631-3635 (2018)

Mortuza, R., Aung, H.L., Taiaroa, G., Opel-Reading, H.K., Cook, G.M. and K.L. Krause. Overexpression of a newly identified d-amino acid transaminase in Mycobacterium smegmatis complements glutamate racemase deletion. Molecular Microbiology 107:198–213 (2018)

Hards, K. and Cook, G.M. Targeting bacterial energetics to produce new antimicrobials. Drug Resistance Updates 36:1–12 (2018)

Aung, H.L., Tun, T., Nyunt, W.W., Fong, Y., Crump, J.A., Thinn, K.K., Aung, S.T. and G.M. Cook. Association between anti-tuberculosis drug resistance-conferring mutations and treatment outcomes in Myanmar. Infectious Diseases (Lond). 50:388-390 (2018)

2017

Cook, G.M.,Hards, K., Dunn, E., Heikal, A., Nakatani, Y., Greening, C., Crick, D.C., Fontes, F.L., Pethe, K., Hasenoehrl, E. and Berney, M. OXPHOS as a target space for tuberculosis: success, caution, and future directions. In Tuberculosis and the Tubercle Bacillus Third Edition, Edited by William R. Jacobs, Jr., Helen McShane, Valerie Mizrahi and Ian Orme. American Society for Microbiology, Washington, DC. Microbiol Spectrum 5(2): doi:10.1128/microbiolspec.

Nakatani, Y., Opel-Reading, H., Merker, M., Machado, D., Andres, S., Kumar, S., Moradigaravand, D., Coll, F., Perdigão, J., Portugal, I., Schön, T., Nair, D., Devi, U., Kohl, T., Beckert, P., Clark, T., Maphalala, G., Khumalo, D., Diel, R., Toit, K., Aung, H.L. Cook, G.M., Parkhill, J., Peacock, S., Swaminathan, S., Viveiros, M., Niemann, S., Krause, K.L., and C. Köser. Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance. Antimicrobial Agents and Chemotherapy 61:e01575-17 (2017)

Schumacher CE, Harris PWR, Ding XB, Krause, B., Wright, T.H., Cook, G.M., Furkert, D.P. and Brimble, M.A. Synthesis and biological evaluation of novel teixobactin analogues. Organic and Biomolecular Chemistry (Royal Society of Chemistry) 15:8755–8760 (2017)

Harris, P.W.R., Cook, G.M., Leung, I.K.H. and Brimble, M.A. An efficient chemical synthesis of lassomycin enabled by an on-resin lactamisation/off-resin methanolysis strategy and preparation of chemical variants. Australian Journal of Chemistry 70:172-183 (2017)

Nakatani, Y., Jiao, W., Aragao, D., Shimaki, Y., Petri, J., Parker, E. and G.M. Cook. Crystal structure of type II NADH:quinone oxidoreductase from Caldalkalibacillus thermarum with an improved resolution to 2.15 Å. Acta Crystallographica Section F Structural Biology Communications 73:541-549 (2017) Cover of Journal

Kalia, N.P., Hasenoehrl, E.J., Ab Rahman, N.B., Koh, V.H., Ang, M.L., Sajorda, D.R., Hards, K., Grüber, G., Alonso, S., Cook, G.M., Berney, M. and Pethe, K. Exploiting the synthetic lethality between terminal respiratory oxidases to kill Mycobacterium tuberculosis and clear host infection. Proceedings of the National Academy of Sciences of the United States of America 114:7426-7431 (2017)

Weimar, M.R., Cheung, J., Dey, D., McSweeney, C., Morrison, M., Kobayashi, Y., Whitman, W.B., Carbone, V., Schofield, L.R., Ronimus, R.S. and Cook, G.M. Development of multi-well plate methods using pure cultures of methanogens to identify new inhibitors for suppressing ruminant methane emissions. Applied and Environmental Microbiology 83:e00396-17 (2017)

Aung, H.L., Nyunt, W.W., Fong, Y., Cook, G.M. and Aung, S.T. First two extensively drug-resistant tuberculosis cases from Myanmar treated with bedaquiline. Clinical Infectious Diseases 2017 Apr 24. doi: 10.1093/cid/cix365. [Epub ahead of print] (2017)

Carere, C.R., Hards, K., Houghton, K.M., Power, J.F., McDonald, B., Collet, C., Gapes, D.J., Sparling, R., Boyd, E.S., Cook, G.M., Greening, C. and Stott, M.B. Mixotrophy drives niche expansion of verrucomicrobial methanotrophs. ISME Journal doi:10.1038/ismej.2017.112

Mulholland, C.V., Ruthe, A., Cursons, R.T., Durrant, R., Karalus, N., Coley, K., Bower, J., Permina, E., Coleman, M.J., Roberts, S.A., Arcus, V.L., Cook, G.M. and Aung, H.L. Rapid molecular diagnosis of the Mycobacterium tuberculosis Rangipo strain responsible for the largest recurring TB cluster in New Zealand. Diagnostic Microbiology and Infectious Disease Mar 23. pii: S0732-8893(17)30106-2. doi: 10.1016/j.diagmicrobio.2017.03.012 [Epub ahead of print]

Nyunt, W.W., Aung, S.T., Cook, G.M. and Aung, H.L. First- and second-line anti tuberculosis drug resistance patterns among previous treatment failure patients in Myanmar. Journal of Global Antimicrobial Resistance 9:34–35 (2017)

Nyunt, W.W., Aung, S.T., Cook, G.M. and Aung, H.L. Evaluation of the genotype MTBDR sl test for detection of second-line drug resistance in drug-resistant Mycobacterium tuberculosis strains in Myanmar. Infectious Diseases 49: 865-866 (2017)

Blaza, J.N., Bridges, H.R., Aragão, D., Dunn, E.A., Heikal, A., Cook, G.M., Nakatani, Y. and Hirst, J. The mechanism of catalysis by type-II NADH:quinone oxidoreductases. Scientific Reports 7:40165 | DOI: 10.1038/srep40165 (2017) PMID: 28067272

2016

Petridis, M., Vickers, C., Robson, J., McKenzie, J.L., Bereza, M., Sharrock, A., Aung, H.L., Arcus, V.L. and Cook G.M. Structure and function of AmtR in Mycobacteriumsmegmatis:Implications for post-transcriptional regulation of urea metabolism through a small antisense RNA. Journal of Molecular Biology 428:4315–4329 (2016) PMID:27640309

McMillan, D.G, Watanabe, R., Ueno, H., Cook, G.M, and Noji, H. Biophysical characterization of a thermoalkaliphilic molecular motor with a high stepping-torque gives insight into evolutionary ATP synthase adaptation. Journal of Biological Chemistry 2016 Sep 13. pii: jbc.M116.743633. [Epub ahead of print] PMID:27624936 

Ferguson, S.F., Cook, G.M., Montgomery, M.G., Leslie, A.G.W. and Walker, J.E. The regulation of the thermoalkaliphilic F1-ATPase from Caldalkalibacillus thermarum. Proceedings of the National Academy of Sciences of the United States of America 2016 2016 Sep 12. pii: 201612035. [Epub ahead of print] PMID:27621435

Kavianinia, I., Kunalingam, L., Harris, P., Cook, G.M. and Brimble, M. Total synthesis and stereochemical revision of the anti-tuberculosis peptaibol trichoderin A. Organic Letters  2016 Aug 5;18(15):3878-81. doi: 10.1021/acs.orglett.6b01886. Epub 2016 Jul 28. PMID: 27467118

Heikal, A., Hards, K., Cheung, C.-Y., Menorca, A., Timmer, M.S., Stocker, B.L. and Cook, G.M. Activation of type II NADH dehydrogenase by quinolinequinones mediates antitubercular cell death. Journal of Antimicrobial Chemotherapy 71:2840-2847 (2016) PMID: 27365187

Cook, G.M. and Poole, R.K. A bacterial oxidase like no other? Science 352: 518-519 (2016) PMID: 27126025

Aung, H.L., Tun, T., Permina, E., Nyunt, W.W., Aung, S.T., Thinn, K.K., Crump, J.A. and Cook, G.M. Draft genome sequences of two drug-resistant Mycobacterium tuberculosis isolates from Myanmar. Genome Announcements 4(5). pii: e00850-16.

Tun, T., Aye, K.S., Nyunt, W.W., Crump, J.A., Nakajima, C., Suzuki, Y., Thinn, K.K., Cook, G.M. and Aung, H.L. Genotypic diversity of Mycobacterium tuberculosis strains in Myanmar. Infectious Disease (Lond). 2016 Sep 19:1-3. [Epub ahead of print] PMID: 27644269

Henderson, G., Cook, G.M. and Ronimus, R.S. Enzyme- and gene-based approaches for developing methanogen-specific compounds to control ruminant methane emissions: a review. Animal Production Science http://dx.doi.org/10.1071/AN15757.

Medini, K., Harris, P.W.R., Menorca, A., Hards, K., Cook, G.M. and Brimble, M.A. Synthesis and activity of a diselenide bond mimetic of theantimicrobial protein caenopore-5. Chemical Science (Manuscript ID: SC-EDG-11-2015-004187) IN PRESS (2016).

Aung, H.L., Thanda, T., Moradigaravand, D., Köser, C., Nyunt, W.W., Aung, S.T., Lwin, T., Thinn, K.K., Crump, J.A., Parkhill, J., Peacock, S.J., Cook, G.M. and Hill, P.C. Whole-genome sequencing of multidrug-resistant Mycobacterium tuberculosis isolates from Myanmar. Journal of Global Antimicrobial Resistance 6:113-117 (2016)

Holyoake, L.V., Hunt, S., Sanguinetti, G., Cook, G.M., Howard, M.J., Rowe, M.L., Poole, R.K. and Shepherd, M. CydDC-mediated reductant export in Escherichia coli controls the transcriptional wiring of energy metabolism and combats nitrosative stress. Biochemistry Journal 473:693–701 (2016)

Dunn, E.A., Cook, G.M. and Heikal, A. Comparison of lipid and detergent enzyme environments for identifying inhibitors of membrane-bound energy-transducing proteins. Journal of Microbiological Methods 120:41-43 (2016)

Dunn, E.A., Cook, G.M. and Heikal, A. Annotated compound data for modulators of detergent-solubilised or lipid-reconstituted respiratory type II NADH dehydrogenase activity obtained by compound library screening. Data Brief 6:275-278 (2016)

2015

Petridis, M., Benjak, A. and Cook, G.M. Defining the nitrogen regulated transcriptome of Mycobacterium smegmatis using continuous culture. BMC Genomics 16:821 DOI 10.1186/s12864-015-2051-x (2015)

Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., Morales, S.E. Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a universal energy source for microbial growth and survival. The ISME Journal 10:761-77 (2015)

Greening, C., Carere, C.R., Rushton-Green, R., Harold, L.K., Hards, K., Taylor, M.C., Morales, S.E., Stott, M.B. and Cook, G.M. Persistence of dominant soil phylum Acidobacteria by trace gas scavenging. Proceedings of the National Academy of Sciences of the United States of America 112:10497–10502 (2015)

Zhang, X.-X., Gauntlett, J., Oldenburg, D., Cook, G.M. and Rainey, P. Role of the transporter-like sensor kinase CbrA in histidine uptake and signal transduction. Journal of Bacteriology 197:2867–2878 (2015)

Lu, P., Heineke, M.H., Koul, A., Andries, K., Cook, G.M., Lill, H., van Spanning, R. and Bald, D. The cytochrome bd-type quinol oxidase is important for survival of Mycobacterium smegmatis under peroxide and antibiotic-induced stress. Scientific Reports 5:10333 | DOI: 10.1038/srep10333 (2015)

Campen, R.L., Ackerley, D.F., Cook, G.M. and O’Toole, R.F. Development of a Mycobacterium smegmatis transposon mutant array for characterising the mechanism of action of tuberculosis drugs: findings with isoniazid and its structural analogues. Tuberculosis (Edinb) 95:432-439 (2015)

Tran, T.T., Palmer, H.R., Weimar, M.R., Arias, C.A., Cook, G.M. and Murray, B.E. Oral bacitracin: a consideration for suppression of intestinal VRE and for VRE bacteremia from an apparent GI tract source. Clinical Infectious Diseases 60:1726-1728 (2015)

Hards, K., Robson, J., Berney, M., Shaw, L., Bald, D., Koul, A., Andries, K. and Cook, G.M. Bactericidal mode of action of bedaquiline. Journal of Antimicrobial Chemotherapy 70:2028–2037 (2015)

Aung, H.L., Dey, D., Janssen, P.H., Ronimus, R.S. and Cook, G.M. A high-throughput screening assay for identification of inhibitors of the A1AO-ATP synthase of the rumen methanogen Methanobrevibacter ruminantium M1. Journal of Microbiological Methods 110:15-17 (2015)

Aung, H.L., Dixon, L.L., Smith, L.J., Sweeney, N.P., Robson, J.R., Berney, M., Buxton, R.S., Green, J. and Cook, G.M. Novel regulatory roles of cAMP receptor proteins (CRPs) in fast-growing environmental mycobacteria. Microbiology 161:648-61 (2015)

 Medini, K., Harris, P.W.R., Hards, K., Dingley, A.J., Cook, G.M. and Brimble, M.A. The chemical synthesis of caenopore-5; a pore forming antimicrobial protein, using native chemical ligation at a Glu-Cys site. ChemBioChem 16:328-336 (2015)

2014

Cook, G.M., Greening, C., Hards, K. and Berney, M. Energetics of pathogenic bacteria and opportunities for drug development. Advances in Microbial Physiology - Advances in Bacterial Pathogen Biology 65:1-62. Epub 2014 Nov 4.

Hartman, T.,Weinrick, B., Vilchèze, C., Berney, M., Tufariello, J., Cook, G.M. and Jacobs, W.R. Jr. Succinate dehydrogenase is the regulator of respiration in Mycobacterium tuberculosis. PLoS Pathogens 10: e1004510 (2014)

Li, Y., Mortuza, R., Milligan, D., Tran, S., Strych, U., Cook, G.M. and Krause, K. Investigation on the essentiality of glutamate racemase in Mycobacterium smegmatis. Journal of Bacteriology Sep 22 [Epub ahead of print] (2014)

Dunn, E.A., Roxburgh, M., Larsen, L., Smith, R.A.J., McLellan, A.D., Heikal, A., Murphy, M.P. and Cook, G.M. Incorporation of triphenylphosphonium functionality improves the inhibitory properties of phenothiazine derivatives in Mycobacterium tuberculosis. Bioorganic & Medicinal Chemistry http://dx.doi.org/10.1016/j.bmc.2014.07.050 (2014)

Berney, M., Greening, C., Conrad, R., Jacobs, W.R. and Cook, G.M. An obligately aerobic soil bacterium activates fermentative hydrogen production to survive reductive stress during hypoxia. Proceedings of the National Academy of Sciences of the United States of America 111:11479–11484 (2014) 

Pecsi, I., Hards, K., Ekanayaka, N., Berney, M., Hartman, T., Jacobs Jr., W.R. and Cook, G.M. Essentiality of succinate dehydrogenase in Mycobacterium smegmatis and its role in the generation of the membrane potential under hypoxia. mBio 5(4):e01093-14 (2014) 

Greening, C., Villas-Bôas, S.G., Robson, J., Berney, M. and Cook, G.M. The growth and survival of Mycobacterium smegmatis is enhanced by co-metabolism of atmospheric H2. PLoS ONE 9(7):e103034 (2014)

Gebhard, S., Busby, J., Fritz, G., Moreland, N., Cook, G.M., Lott, S., Baker, E.N. and Money, V. Crystal structure of PhnF, a GntR-family transcriptional regulator of phosphate transport in Mycobacterium smegmatis. Journal of Bacteriology 196:3472–3481 (2014)

Aung, H.L., Berney, M. and Cook, G.M. Hypoxia-activated cytochrome bd expression in Mycobacterium smegmatis is cAMP receptor protein (CRP)-dependent.Journal of Bacteriology 196:3091-3097 (2014)

Kingston, A.W., Zhao, H., Cook, G.M. and Helmann, J.D. Accumulation of heptaprenyl diphosphate sensitizes Bacillus subtilis to bacitracin: Implications for the mechanism of resistance mediated by the BceAB transporter. Molecular Microbiology 93:37-49 (2014)

Portela, C., Smart, K., Tumanov, S., Cook, G.M. and Villas-Boas, S.* The global metabolic response of Enterococcus faecalis to oxygen. Journal of Bacteriology 96:2012-2022 (2014)

Fang, C., Stiegeler, E., Cook, G.M., Mascher, T. and Gebhard, S.*  Bacillus subtilis as a platform for molecular characterisation of regulatory mechanisms of Enterococcus faecalis resistance against cell wall antibiotics. PLoS ONE 9:e93169 (2014) 

Arraiano, C.M. and Cook, G.M. Editorial overview: Cell regulation: Microbial cell regulation-looking in from the outside. Current Opinion in Microbiology 18:1–3 (2014)

Greening, C., Berney, M., Hards, K., Cook, G.M.*, Conrad, R.* A soil actinobacterium scavenges atmospheric H2 using two membrane-associated, oxygen-dependent [NiFe]-hydrogenases. Proceedings of the National Academy of Sciences of the United States of America 111:4257–4261 (2014)

Greening, C.* and Cook, G.M.* Integration of hydrogenase expression and hydrogen sensing in bacterial cell physiology.  Current Opinion in Microbiology 18:30–38 (2014)

Cook, G.M.*, Hards, K., Vilchèze, C., Hartman, Y. and Berney, M. Energetics of Respiration and Oxidative Phosphorylation in Mycobacteria. In Molecular Genetics of Mycobacteria, Hatfull, G. and Jacobs, Jr., W.R. (Eds) ASM Press, Washington DC. IN PRESS (2014).

Heikal, A., Nakatani, Y., Dunn, E., Weimar, M.R., Day, C., Baker, E.N., Lott, J.S., Sazanov, L.A. and Cook, G.M.* Structure of the bacterial type II NADH dehydrogenase: a monotopic membrane protein with an essential role in energy generation. Molecular Microbiology 91:950–964 (2014) Cover of Journal

Lagautriere, T., Bashiri, G., Paterson, N., Berney, M., Cook, G.M. and Baker, E.N.* Characterization of the proline utilization pathway in Mycobacterium tuberculosis through structural and functional studies. Acta Crystallographica Section D70, 968–980 (2014)

Berney, M., Greening, C., Hards, K., Collins, D.M. and Cook, G.M.* Three different [NiFe]-hydrogenases confer metabolic flexibility in the obligate aerobe Mycobacterium smegmatis. Environmental Microbiology 16:318–330 (2014)

Gebhard, S.*, Fang, C., Shaaly, A., Leslie, D., Weimar, M., Kalamorz, F., Carne, A., and Cook, G.M. Identification and characterisation of a bacitracin resistance network in Enterococcus faecalis. Antimicrobial Agents and Chemotherapy 58:1425-1433 (2014)

McNeil, M.B., Hampton, H.G., Hards, K.J. Watson, B.N.J., Cook, G.M. and Fineran, P.C. The succinate dehydrogenase assembly factor, SdhE, is required for the flavinylation and activation of fumarate reductase in bacteria. FEBS Letters 588:414-421 (2014)


Previous group members 

Postdoctoral or Research Fellows

Dr Roman Mortuza, 2017-18

Dr Jess Petri, 2017

Dr Michael Petridis, 2015-2017

Dr Adam Heikal, 2010-2016

Dr Ildiko Pecsi, 2012-2016

Dr Jennifer Robson, 2010-2014

Dr Michael Berney, 2007-2012

Dr Naohiro Taniguichi, 2009-2012

Dr Falk Kalamorz, 2009-2011

Dr Jonathan Gauntlett, 2008

Dr Duncan McMillan, 2008-2009

Dr Scott Ferguson, 2007-2008

Dr Susanne Gebhard, 2006 – 2009

Dr Sunil Ratnayake, 2003

Dr Stefanie Keis, 2001 – 2008

Sieu Tran, 2000-2007

PhD students

Rachel Darnell 2014-17

Kiel Hards, 2014-17

Jessica Petri, 2013–16

Michael Petridis, 2012-15

Elyse Dunn, 2012-15

Chris Greening, 2010-14 

Marina Iglesias Cans, 2010-14

Htin Lin Aung, 2010-13

Aishath Shaaly, 2006-13

Nandula Ekanayaka, 2008-13

Rebekah Frampton, 2008-11

Jennifer Robson, 2005-2010

Anja Humpel, 2005-2010

Sui Mae Lee, 2004-2009

Pearl Swe, 2004-2008

Duncan McMillan, 2004-2008

Jonathan Gauntlett, 2004-2008

Rebecca McDougal, 2003-2007

Scott Ferguson, 2003-2007

Susanne Gebhard, 2003-2006

Matthew James Shirley, 1999-2006

Rajan Prasad Adhikari, 2000-2004

Karen Olsson, 2001-2004

Janet Manson, 2000-2004

MSc students

Melanie Knottenbelt 2016-18

Lisa Shaw, 2008-2010

Marion R. Weimar, 2009

Faith Cox, 2004-2006

Pearl Swe, 2001-2003

Cameron Simmers, 2001-2003

Ian Monk, 2001-2003

Richard Udy, 1999-2001

Connor Wright, 2000-2002

Rebecca McDougal, 2000-2002

Craig Anderson, 1999-2001

Katherine A. Young 1998-2000

Kere R. Kobayashi, 1998-1999