I work with Dr Bruce Russell on malaria. Vivax Malaria is the most widely distributed cause of human malaria. It is also the most and difficult to diagnose and treat. Our work focuses on the biology of the parasite Plasmodium and how we can kill it using therapeutics or vaccinations. To do this we have developed key ex vivo tools and methods to examine P. vivax drug susceptibility and reticulocyte invasion.
Chua, A. C. Y., Ong, J. J. Y., Malleret, B., Suwanarusk, R., Kosaisavee, V., Zeeman, A.-M., … Russell, B., & Bifani, P. (2019). Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages. Nature Communications, 10, 3635. doi: 10.1038/s41467-019-11332-4
Cravo, P., Machado, R. B., Leite, J. A., Leda, T., Suwanarusk, R., Bittencourt, N., … Russell, B., … Costa, F. T. M. (2018). In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate. Malaria Journal, 17, 20. doi: 10.1186/s12936-017-2144-x
Chu, T. T. T., Sinha, A., Malleret, B., Suwanarusk, R., Park, J. E., Naidu, R., … Russell, B., & Chandramohanadas, R. (2018). Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. British Journal of Haematology, 180(1), 118-133. doi: 10.1111/bjh.14976
Basilico, N., Parapini, S., Sparatore, A., Romeo, S., Misiano, P., Vivas, L., … Russell, B., Suwanarusk, R., … Taramelli, D. (2017). In vivo and in vitro activities and ADME-Tox profile of a quinolizidine-modified 4-aminoquinoline: A potent anti-P. falciparum and Anti-P. vivax blood-stage antimalarial. Molecules, 22(12), 2102. doi: 10.3390/molecules22122102
Kosaisavee, V., Suwanarusk, R., Chua, A. C. Y., Kyle, D. E., Malleret, B., Zhang, R., … Ong, J. J. Y., … Russell, B. (2017). Strict tropism for CD71+/CD234+ human reticulocytes limits the zoonotic potential of Plasmodium cynomolgi. Blood, 130(11), 1357-1363. doi: 10.1182/blood-2017-02-764787
Bancone, G., Malleret, B., Suwanarusk, R., Cowwiwat, N., Chu, C. S., McGready, R., … Russell, B. (2017). Asian G6PD-Mahidol reticulocytes sustain normal Plasmodium vivax development. Journal of Infectious Diseases, 216(2), 263-266. doi: 10.1093/infdis/jix278
Zhang, R., Suwanarusk, R., Malleret, B., Cooke, B. M., Nosten, F., Lau, Y.-L., … Russell, B. (2016). A basis for rapid clearance of circulating ring-stage malaria parasites by the spiroindolone KAE609. Journal of Infectious Diseases, 213(1), 100-104. doi: 10.1093/infdis/jiv358
Auburn, S., Serre, D., Pearson, R. D., Amato, R., Sriprawat, K., To, S., … Suwanarusk, R., Russell, B., … Price, R. N. (2016). Genomic analysis reveals a common breakpoint in amplifications of the Plasmodium vivax multidrug resistance 1 locus in Thailand. Journal of Infectious Diseases, 214(8), 1235-1242. doi: 10.1093/infdis/jiw323
Zhang, R., Lee, W.-C., Lau, Y.-L., Albrecht, L., Lopes, S. C. P., Costa, F. T. M., Suwanarusk, R., … Russell, B. (2016). Rheopathologic consequence of Plasmodium vivax rosette formation. PLoS Neglected Tropical Diseases, 10(8), e0004912. doi: 10.1371/journal.pntd.0004912
Teng, Y., Suwanarusk, R., Ngai, M. H., Srinivasan, R., Ong, A. S. M., Ho, B., … Chai, C. L. L. (2015). An amidation/cyclization approach to the synthesis of N-hydroxyquinolinones and their biological evaluation as potential anti-plasmodial, anti-bacterial, and iron(II)-chelating agents. Bioorganic & Medicinal Chemistry Letters, 25(3), 607-610. doi: 10.1016/j.bmcl.2014.12.014
de Cassan, S. C., Shakri, A. R., Llewellyn, D., Elias, S. C., Cho, J. S., Goodman, A. L., … Suwanarusk, R., … Russell, B. M., … Draper, S. J. (2015). Preclinical assessment of viral vectored and protein vaccines targeting the Duffy-binding protein region II of Plasmodium vivax. Frontiers in Immunology, 6, 348. doi: 10.3389/fimmu.2015.00348
Malleret, B., Li, A., Zhang, R., Tan, K. S. W., Suwanarusk, R., Claser, C., … Russell, B. (2015). Plasmodium vivax: Restricted tropism and rapid remodeling of CD71-positive reticulocytes. Blood, 125(8), 1314-1324. doi: 10.1182/blood-2014-08-596015
Malmquist, N. A., Sundriyal, S., Caron, J., Chen, P., Witkowski, B., Menard, D., Suwanarusk, R., … Scherf, A. (2015). Histone methyltransferase inhibitors are orally bioavailable, fast-acting molecules with activity against different species causing malaria in humans. Antimicrobial Agents & Chemotherapy, 59(2), 950-959. doi: 10.1128/AAC.04419-14
Suwanarusk, R., Russell, B., Ong, A., Sriprawat, K., Chu, C. S., PyaePhyo, A., … Renia, L. (2015). Methylene blue inhibits the asexual development of vivax malaria parasites from a region of increasing chloroquine resistance. Journal of Antimicrobial Chemotherapy, 70(1), 124-129. doi: 10.1093/jac/dku326
Lee, W.-C., Malleret, B., Lau, Y.-L., Mauduit, M., Fong, M.-Y., Cho, J. S., Suwanarusk, R., … Russell, B. (2014). Glycophorin C (CD236R) mediates vivax malaria parasite rosetting to normocytes. Blood, 123(18), e100-e109. doi: 10.1182/blood-2013-12-541698
Kuhen, K. L., Chatterjee, A. K., Rottmann, M., Gagaring, K., Borboa, R., Buenviaje, J., … Russell, B., Suwanarusk, R., … Diagana, T. T. (2014). KAF156 is an antimalarial clinical candidate with potential for use in prophylaxis, treatment, and prevention of disease transmission. Antimicrobial Agents & Chemotherapy, 58(9), 5060-5067. doi: 10.1128/AAC.02727-13
Loh, C. C.-Y., Suwanarusk, R., Lee, Y. Q., Chang, K. W. K., Choy, K.-Y., Rénia, L., Russell, B., … Chow, L. M. C. (2014). Characterization of the commercially-available fluorescent chloroquine-BODIPY conjugate, LynxTag- CQGREEN, as a marker for chloroquine resistance and uptake in a 96-well plate assay. PLoS ONE, 9(10), e0110800. doi: 10.1371/journal.pone.0110800
McNamara, C. W., Lee, M. C. S., Lim, C. S., Lim, S. H., Roland, J., Nagle, A., … Suwanarusk, R., Russell, B., … Winzeler, E. A. (2013). Targeting Plasmodium PI(4)K to eliminate malaria. Nature, 504(7479), 248-253. doi: 10.1038/nature12782
Lee, W.-C., Russell, B., Lau, Y.-L., Fong, M.-Y., Chu, C., Sriprawat, K., … Suwanarusk, R., … Renia, L. (2013). Giemsa-stained wet mount based method for reticulocyte quantification: A viable alternative in resource limited or malaria endemic settings. PLoS ONE, 8(4), e60303. doi: 10.1371/journal.pone.0060303
Malleret, B., Xu, F., Mohandas, N., Suwanarusk, R., Chu, C., Leite, J. A., … Russell, B. (2013). Significant biochemical, biophysical and metabolic diversity in circulating human cord blood reticulocytes. PLoS ONE, 8(10), e76062. doi: 10.1371/journal.pone.0076062
Koh, S., Shimasaki, N., Suwanarusk, R., Ho, Z. Z., Chia, A., Banu, N., … Bertoletti, A. (2013). A practical approach to immunotherapy of hepatocellular carcinoma using T cells redirected against hepatitis B virus. Molecular Therapy: Nucleic Acids, 2, e114. doi: 10.1038/mtna.2013.43
Russell, B., Malleret, B., Suwanarusk, R., Anthony, C., Kanlaya, S., Lau, Y. L., … Renia, L. (2013). Field-based flow cytometry for ex vivo characterization of Plasmodium vivax and P. falciparumantimalarial sensitivity. Antimicrobial Agents & Chemotherapy, 57(10), 5170-5174. doi: 10.1128/AAC.00682-13
Russell, B., Suwanarusk, R., Malleret, B., Costa, F. T. M., Snounou, G., Baird, J. K., … Rénia, L. (2012). Human ex vivo studies on asexual Plasmodium vivax: The best way forward. International Journal for Parasitology, 42(12), 1063-1070. doi: 10.1016/j.ijpara.2012.08.010
Rénia, L., Howland, S. W., Claser, C., Gruner, A. C., Suwanarusk, R., Teo, T.-H., Russell, B., & Ng, L. F. P. (2012). Cerebral malaria: Mysteries at the blood-brain barrier. Virulence, 3(2), 193-201. doi: 10.4161/viru.19013
Chotivanich, K., Udomsangpetch, R., Suwanarusk, R., Pukrittayakamee, S., Wilairatana, P., Beeson, J. G., … White, N. J. (2012). Plasmodium vivax adherence to placental glycosaminoglycans. PLoS ONE, 7(4), e34509. doi: 10.1371/journal.pone.0034509
Borlon, C., Russell, B., Sriprawat, K., Suwanarusk, R., Erhart, A., Renia, L., … D'Alessandro, U. (2012). Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture. International Journal for Parasitology, 42(2), 155-160. doi: 10.1016/j.ijpara.2011.10.011
Kosaisavee, V., Lek-Uthai, U., Suwanarusk, R., Grüner, A. C., Russell, B., Nosten, F., … Snounou, G. (2012). Genetic diversity in new members of the reticulocyte binding protein family in Thai Plasmodium vivax isolates. PLoS ONE, 7(3), e32105. doi: 10.1371/journal.pone.0032105
Kaewpongsri, S., Sriprawat, K., Suwanarusk, R., Kyle, D. E., Lek-Uthai, U., Leimanis, M., … Russell, B., … Renia, L. (2011). The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum. Antimicrobial Agents & Chemotherapy, 55(3), 1300-1304. doi: 10.1128/AAC.01103-10
Prajapati, S. K., Joshi, H., Shalini, S., Patarroyo, M. A., Suwanarusk, R., Kumar, A., … Dash, A. P. (2011). Plasmodium vivax lineages: Geographical distribution, tandem repeat polymorphism, and phylogenetic relationship. Malaria Journal, 10, 374. doi: 10.1186/1475-2875-10-374
Malleret, B., Claser, C., Ong, A. S. M., Suwanarusk, R., Sriprawat, K., Howland, S. W., Russell, B., … Rénia, L. (2011). A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development. Scientific Reports, 1, 118. doi: 10.1038/srep00118
Russell, B., Suwanarusk, R., Borlon, C., Costa, F. T. M., Chu, C. S., Rijken, M. J., … Rénia, L. (2011). A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax. Blood, 118(13), e74-e81. doi: 10.1182/blood-2011-04-348748
Proux, S., Suwanarusk, R., Barends, M., Zwang, J., Price, R. N., Leimanis, M., … Russell, B., … Snounou, G. (2011). Considerations on the use of nucleic acid-based amplification for malaria parasite detection. Malaria Journal, 10, 323. doi: 10.1186/1475-2875-10-323
Carvalho, B. O., Lopes, S. C. P., Nogueira, P. A., Orlandi, P. P., Bargieri, D. Y., Blanco, Y. C., … Russell, B., Suwanarusk, R., … Costa, F. T. M. (2010). On the cytoadhesion of Plasmodium vivax—infected erythrocytes. Journal of Infectious Diseases, 202(4), 638-647. doi: 10.1086/654815
Leimanis, M. L., Jaidee, A., Sriprawat, K., Kaewpongsri, S., Suwanarusk, R., Barends, M., … Russell, B., … Nosten, F. (2010). Plasmodium vivax susceptibility to ferroquine. Antimicrobial Agents & Chemotherapy, 54(5), 2228-2230. doi: 10.1128/AAC.01572-09
Rottmann, M., McNamara, C., Yeung, B. K. S., Lee, M. C. S., Zou, B., Russell, B., … Suwanarusk, R., … Diagana, T. T. (2010). Spiroindolones, a potent compound class for the treatment of malaria. Science, 329(5996), 1175-1180. doi: 10.1126/science.1193225
Sriprawat, K., Kaewpongsri, S., Suwanarusk, R., Leimanis, M. L., Lek-Uthai, U., Phyo, A. P., … Russell, B., … Nosten, F. (2009). Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood. Malaria Journal, 8, 115. doi: 10.1186/1475-2875-8-115
Lek-Uthai, U., Suwanarusk, R., Ruengweerayut, R., Skinner-Adams, T. S., Nosten, F., Gardiner, D. L., … Russell, B. (2008). Stronger activity of human immunodeficiency virus type 1 protease inhibitors against clinical isolates of Plasmodium vivax than against those of P. falciparum. Antimicrobial Agents & Chemotherapy, 52(7), 2435-2441. doi: 10.1128/AAC.00169-08
Russell, B., Chalfein, F., Prasetyorini, B., Kenangalem, E., Piera, K., Suwanarusk, R., … Price, R. N. (2008). Determinants of in vitro drug susceptibility testing of Plasmodium vivax. Antimicrobial Agents & Chemotherapy, 52(3), 1040-1045. doi: 10.1128/AAC.01334-07
Sharrock, W. W., Suwanarusk, R., Lek-Uthai, U., Edstein, M. D., Kosaisavee, V., Travers, T., … Russell, B. (2008). Plasmodium vivax trophozoites insensitive to chloroquine. Malaria Journal, 7, 94. doi: 10.1186/1475-2875-7-94
Suwanarusk, R., Chavchich, M., Russell, B., Jaidee, A., Chalfein, F., Barends, M., … Price, R. N. (2008). Amplification of pvmdr1 associated with multidrug-resistant Plasmodium vivax. Journal of Infectious Diseases, 198(10), 1558-1564. doi: 10.1086/592451
Suwanarusk, R., Russell, B., Chavchich, M., Chalfein, F., Kenangalem, E., Kosaisavee, V., … Price, R. N. (2007). Chloroquine resistant Plasmodium vivax: In vitro characterisation and association with molecular polymorphisms. PLoS ONE, 2(10), e1089. doi: 10.1371/journal.pone.0001089
Boonma, P., Christensen, P. R., Suwanarusk, R., Price, R. N., Russell, B., & Lek-Uthai, U. (2007). Comparison of three molecular methods for the detection and speciation of Plasmodium vivax and Plasmodium falciparum. Malaria Journal, 6, 124. doi: 10.1186/1475-2875-6-124
Kosaisavee, V., Suwanarusk, R., Nosten, F., Kyle, D. E., Barrends, M., Jones, J., … Russell, B., & Lek-Uthai, U. (2006). Plasmodium vivax: Isotopic, PicoGreen, and microscopic assays for measuring chloroquine sensitivity in fresh and cryopreserved isolates. Experimental Parasitology, 114(1), 34-39. doi: 10.1016/j.exppara.2006.02.006
Imwong, M., Russell, B., Suwanarusk, R., Nzila, A., Leimanis, M. L., Sriprawat, K., … Renia, L. (2011). Methotrexate is highly potent against pyrimethamine-resistant Plasmodium vivax [Brief report]. Journal of Infectious Diseases, 203(2), 207-210. doi: 10.1093/infdis/jiq024
Russell, B., Suwanarusk, R., & Lek-Uthai, U. (2006). Plasmodium vivax genetic diversity: Microsatellite length matters [Update]. Trends in Parasitology, 22(9), 399-401. doi: 10.1016/j.pt.2006.06.013
Suwanarusk, R., Cooke, B. M., Dondorp, A. M., Silamut, K., Sattabongkot, J., White, N. J., & Udomsangpetch, R. (2004). The deformability of red blood cells parasitized by Plasmodium falciparum and P. vivax. Journal of Infectious Diseases, 189(2), 190-194. doi: 10.1086/380468
