Mycobacterum tuberculosis, the causative agent of tuberculosis (TB), is an obligate human pathogen and leading cause of death by a single infectious agent. By studying drug resistance, bacterial metabolism, and interactions between drug targets the goal of our work is to drive the development of novel therapeutics and treatment strategies to combat this global health problem.
This research includes microbiology, molecular biology, next-generation sequencing, antimicrobial testing and PC3 laboratory experiments.
Co-Supervised Post Graduate Students
Maurice Wilkins Centre for Molecular Biodiscovery
Marsden Fund (RSNZ)
2015-2018: Postdoctoral Scientist, Infectious Disease Research Institute, Seattle, USA.
2013-2015: Postdoctoral Scientist, Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
2012-2013: Postdoctoral Scientist, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
With MSc student Natalie Waller
Google Scholar: https://scholar.google.co.nz/citations?user=aI1eYDsAAAAJ&hl=en
* Denotes joint first author. # Denotes corresponding Author
Hards, K. Adolph, C. Harold, LK. McNeil, MB. Cheung, CY. Jinich, A. Rhee, KY. and Cook, GM. 2019. Two for the price of one: Attacking the energetic-metabolic hub of mycobacteria to produce new chemotherapeutic agents. Prog Biophys Mol Biol. 13.
McNeil MB# and Cook GM. 2019. Utilization of CRISPR interference to validate MmpL3 as a drug target in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy. 63(8)
McNeil MB, Chettiar S, Awasthi D and Parish T. 2019. Cell wall inhibitors increase the accumulation of rifampicin in Mycobacterium tuberculosis. Access Microbiology. 1(1).
Robertson GT, Ektnitphong VA, Scherman MS, McNeil MB + 13 Authors and Lenaerts AJ. 2019. Efficacy and improved resistance potential of a cofactor-independent InhA inhibitor of Mycobacterium tuberculosis in a C3HeB/FeJ mouse model with advanced lung pathology. Antimicrobial Agents and Chemotherapy. 63(4).
Xia Yi, Zhou Y, Carter DS, McNeil MB + 19 Authors and Alley MRK. 2018. Discovery of a cofactor-independent inhibitor of Mycobacterium tuberculosis InhA. Life Science Alliance. 1(3). e20180025.
McNeil MB, Dennison D, Shelton C, Flint L, Korkegian A and Parish T. 2017. Mechanisms of resistance against NITD-916, a direct inhibitor of Mycobacterium tuberculosis InhA. Tuberculosis.107. 133-136.
McNeil MB, Dennison D, Shelton C and Parish T. 2017. In vitro isolation and characterisation of oxazolidinone resistant Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy.61(10). e01296-17.
McNeil MB, Dennison D and Parish T. 2017. Mutations in MmpL3 alter membrane potential, hydrophobicity and antibiotic susceptibility in Mycobacterium smegmatis. Microbiology. 163. 1065-1070.
Hampton HG, McNeil MB*, Paterson TJ, Ney B, Williamson NR, Easingwood RA, Bostina M, Salmond GP, Fineran PC. 2016. CRISPR-Cas gene-editing reveals RsmA and RsmC act through FlhDC to repress the SdhE flavinylation factor and control motility and prodigiosin production in Serratia. Microbiology. 162(2). 1047-58.
Richter C, Dy RL, Mckenzie RE, Watson BNJ, Taylor C, Chang JT, McNeil MB, Staals RHJ and Fineran PC. 2014. Priming in the Type 1-F CRISPR-Cas system triggers hyperactive strand independent spacer acquisition nearby the primed protospacer. Nucleic Acids Research. 42(13). 8516-26.
McNeil MB, Hampton HG, Hards KJ, Watson BNJ, Cook GM and Fineran PC. 2014. The succinate dehydrogenase assembly factor, SdhE, is required for the flavinylation and activation of fumarate reductase in bacteria. FEBS Letters. 588(3): 414-421.
Fineran PC, +12 other authors. 2013. Draft genome sequence of Serratia sp. strain ATCC 39006, a model bacterium for analysis of the biosynthesis and regulation of prodigiosin, a carbapenem and gas vesicles. Genome Announcements. 1(6): e0139-13.
McNeil MB and Fineran PC. 2013. The conserved RGxxE motif of the bacterial FAD assembly factor SdhE is required for succinate dehydrogenase flavinylation and activity. Biochemistry. 52(43): 7628-7640.
McNeil MB, Iglesias Cans M, Clulow JS and Fineran PC. 2013. YgfX (CptA) is a multimeric membrane protein that interacts with the succinate dehydrogenase assembly factor, SdhE (YgfY). Microbiology. 159: 1352-1365.
McNeil MB, and Fineran PC. 2012. Prokaryotic assembly factors for the attachment of flavins to complex II. BBA Bioenergetics. 1827(5): 637-647.
McNeil MB, Clulow JS, Wilf N, Salmond GPC and Fineran PC. 2012. SdhE is a conserved protein required for the flavinylation of succinate dehydrogenase in bacteria. Journal of Biological Chemistry. 287(22): 18418-18428.
Gristwood T, McNeil MB, Clulow JS, Salmond GPC and Fineran PC. 2011. PigS and PigP Regulate Prodigiosin Biosynthesis in Serratia via Differential Control of Divergent Operons, Which Include Predicted Transporters of Sulfur-Containing Molecules. Journal of Bacteriology. 193:1076-1085.