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Microbiology Logo Microbiology & Immunology
Te Tari Moromoroiti me te Ārai Mate

Dr John Sullivan


Research interests:

Bacterial and viral genetics

Current research:

My research focuses on Rhizobium Bacterial and Viral Genetics, including: defining the minimal microsymbiont complement and novel plant genes required to build a root nodule; functional genomics of the Mesorhizobium symbiosis island; microbial genomics; and Rhizobia and sustainable agriculture.

Publications:

Verdonk, C. J., Sullivan, J. T., Williman, K. M., Nicholson, L., Bastholm, T. R., Hynes, M. F., Ronson, C. W., … Ramsay, J. P. (2019). Delineation of the integrase-attachment and origin-of-transfer regions of the symbiosis island ICEMlSymR7APlasmid104, 102416.  doi: 10.1016/j.plasmid.2019.102416

Kelly, S., Sullivan, J. T., Kawaharada, Y., Radutoiu, S., Ronson, C. W., & Stougaard, J. (2018). Regulation of Nod factor biosynthesis by alternative NodD proteins at distinct stages of symbiosis provides additional compatibility scrutiny. Environmental Microbiology20(1), 97-110.  doi: 10.1111/1462-2920.14006

Haskett, T. L., Ramsay, J. P., Bekuma, A. A., Sullivan, J. T., O'Hara, G. W., & Terpolilli, J. J. (2017). Evolutionary persistence of tripartite integrative and conjugative elements. Plasmid92, 30-36.  doi: 10.1016/j.plasmid.2017.06.001

Haskett, T. L., Terpolilli, J. J., Bekuma, A., O'Hara, G. W., Sullivan, J. T., Wang, P., Ronson, C. W., & Ramsay, J. P. (2016). Assembly and transfer of tripartite integrative and conjugative genetic elements. PNAS113(42), 12268-12273.  doi: 10.1073/pnas.1613358113

Muszyński, A., Heiss, C., Hjuler, C. T., Sullivan, J. T., Kelly, S. J., Thygesen, M. B., … Ronson, C. W. (2016). Structures of exopolysaccharides involved in receptor-mediated perception of Mesorhizobium lotiby Lotus japonicusJournal of Biological Chemistry291(40), 20946-20961.  doi: 10.1074/jbc.M116.743856

Ramsay, J. P., Tester, L. G. L., Major, A. S., Sullivan, J. T., Edgar, C. D., Kleffmann, T., … Tate, W. P., … Ronson, C. W. (2015). Ribosomal frameshifting and dual-target antiactivation restrict quorum-sensing–activated transfer of a mobile genetic element. PNAS112(3), 4104-4109.  doi: 10.1073/pnas.1501574112

Reeve, W., Sullivan, J., Ronson, C., Tian, R., Munk, C., Han, C., … Kyrpides, N. (2015). High-quality draft genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain CJ3Sym. Standards in Genomic Sciences10, 54.  doi: 10.1186/s40793-015-0049-2

Maolanon, N. N., Blaise, M., Sørensen, K. K., Thygesen, M. B., Cló, E., Sullivan, J. T., Ronson, C. W., … Jensen, K. J. (2014). Lipochitin oligosaccharides immobilized through oximes in glycan microarrays bind LysM proteins. ChemBioChem15(3), 425-434.  doi: 10.1002/cbic.201300520

Kelly, S., Sullivan, J., Ronson, C., Tian, R., Bräu, L., Davenport, K., … Reeve, W. (2014). Genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain NZP2037. Standards in Genomic Sciences9, 7.  doi: 10.1186/1944-3277-9-7

Reeve, W., Sullivan, J., Ronson, C., Tian, R., Bräu, L., Davenport, K., … Kyrpides, N. (2014). Genome sequence of the Lotus corniculatus microsymbiont Mesorhizobium loti strain R88B. Standards in Genomic Sciences9, 3.  doi: 10.1186/1944-3277-9-3

Östermann, J., Marsh, J., Laine, P. K., Zeng, Z., Alatalo, E., Sullivan, J. T., … Lindström, K. (2014). Genome sequencing of two Neorhizobium galegae strains reveals a noeT gene responsible for the unusual acetylation of the nodulation factors. BMC Genomics15(1), 500.  doi: 10.1186/1471-2164-15-500

Kelly, S., Sullivan, J., Ronson, C., Tian, R., Bräu, L., Munk, C., … Reeve, W. (2014). Genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain R7A. Standards in Genomic Sciences9, 6. doi: 10.1186/1944-3277-9-6

Sullivan, J. T., Brown, S. D., & Ronson, C. W. (2013). The NifA-RpoN regulon of Mesorhizobium lotistrain R7A and its symbiotic activation by a novel LacI/GalR-family regulator. PLoS ONE8(1), e53762.  doi: 10.1371/journal.pone.0053762

Ramsay, J. P., Major, A. S., Komarovsky, V. M., Sullivan, J. T., Dy, R. L., Hynes, M. F., … Ronson, C. W. (2013). A widely conserved molecular switch controls quorum sensing and symbiosis island transfer in Mesorhizobium loti through expression of a novel antiactivator. Molecular Microbiology87(1), 1-13.  doi: 10.1111/mmi.12079

Kelly, S. J., Muszyński, A., Kawaharada, Y., Hubber, A. M., Sullivan, J. T., Sandal, N., … Ronson, C. W. (2013). Conditional requirement for exopolysaccharide in the Mesorhizobium-Lotus symbiosis. Molecular Plant-Microbe Interactions26(3), 319-329.  doi: 10.1094/mpmi-09-12-0227-r

Broghammer, A., Krusell, L., Blaise, M., Sauer, J., Sullivan, J. T., Maolanon, N., … Ronson, C. W., … Stougaard, J. (2012). Legume receptors perceive the rhizobial lipochitin oligosaccharide signal molecules by direct binding. PNAS109(34), 13859-13864.  doi: 10.1073/pnas.1205171109

Madsen, L. H., Tirichine, L., Jurkiewicz, A., Sullivan, J. T., Heckmann, A. B., Bek, A. S., Ronson, C. W., … Stougaard, J. (2010). The molecular network governing nodule organogenesis and infection in the model legume Lotus japonicusNature Communications1.  doi: 10.1038/ncomms1009

Ott, T., Sullivan, J., James, E. K., Flemetakis, E., Günther, C., Gibon, Y., Ronson, C., & Udvardi, M. (2009). Absence of symbiotic leghemoglobins alters bacteroid and plant cell differentiation during development of Lotus japonicus root nodules. Molecular Plant-Microbe Interactions22(7), 800-808. doi: 10.1094/MPMI-22-7-0800

Rodpothong, P., Sullivan, J. T., Songsrirote, K., Sumpton, D., Cheung, K. W. J.-T., Thomas-Oates, J., … Ronson, C. W. (2009). Nodulation gene mutants of Mesorhizobium loti R7A—nodZ and nolL mutants have host-specific phenotypes on Lotus spp. Molecular Plant-Microbe Interactions22(12), 1546-1554.  doi: 10.1094/MPMI-22-12-1546

Ramsay, J. P., Sullivan, J. T., Jambari, N., Ortori, C. A., Heeb, S., Williams, P., … Lamont, I. L., & Ronson, C. W. (2009). A LuxRI-family regulatory system controls excision and transfer of the Mesorhizobium loti strain R7A symbiosis island by activating expression of two conserved hypothetical genes. Molecular Microbiology73(6), 1141-1155.  doi: 10.1111/j.1365-2958.2009.06843.x

Miller, S. H., Elliot, R. M., Sullivan, J. T., & Ronson, C. W. (2007). Host-specific regulation of symbiotic nitrogen fixation in Rhizobium leguminosarum biovar trifoliiMicrobiology153, 3184-3195. 

Hubber, A. M., Sullivan, J. T., & Ronson, C. W. (2007). Symbiosis-induced cascade regulation of the Mesorhizobium loti R7A VirB/D4 type IV secretion system. Molecular Plant-Microbe Interactions20(3), 255-261. 

Carlton, T. M., Sullivan, J. T., Stuart, G. S., Hutt, K., Lamont, I. L., & Ronson, C. W. (2007). Ferrichrome utilization in a mesorhizobial population: Microevolution of a three-locus system. Environmental Microbiology9(12), 2923-2932. 

Ramsay, J. P., Sullivan, J. T., Stuart, G. S., Lamont, I. L., & Ronson, C. W. (2006). Excision and transfer of the Mesorhizobium loti R7A symbiosis island requires an integrase IntS, a novel recombination directionality factor RdfS, and a putative relaxase RlxS. Molecular Microbiology62(3), 723-734.  doi: 10.1111/j.1365-2958.2006.05396.x

Hubber, A., Vergunst, A. C., Sullivan, J. T., Hooykaas, P. J. J., & Ronson, C. W. (2004). Symbiotic phenotypes and translocated effector proteins of the Mesorhizobium loti strain R7A VirB/D4 type IV secretion system. Molecular Microbiology54(2), 561-574. 

Sullivan, J. T., Trzebiatowski, J. R., Cruickshank, R. W., Gouzy, J., Brown, S. D., Elliot, R. M., Fleetwood, D. J., McCallum, N. G., … Stuart, G. S., Weaver, J. E., Webby, R. J., … Ronson, C. W. (2002). Comparative sequence analysis of the symbiosis island of Mesorhizobium loti strain R7A. Journal of Bacteriology184(11), 3086-3095.  doi: 10.1128/JB.184.11.3086-3095.2002 

Keis, S., Sullivan, J. T., & Jones, D. T. (2001). Physical and genetic map of the Clostridium saccharobutylicum (formerly Clostridium acetobutylicum) NCP 262 chromosome. Microbiology147, 1909-1922. 

Sullivan, J. T., Brown, S. D., Yocum, R. R., & Ronson, C. W. (2001). The bio operon on the acquired symbiosis island of Mesorhizobium sp. strain R7A includes a novel gene involved in pimeloyl-CoA synthesis. Microbiology147, 1315-1322. 

Sullivan, J. T., & Ronson, C. W. (1998). Evolution of Rhizobia by acquisition of a 500-kb symbiosis island that integrates into a phe-tRNA gene. PNAS95, 5145-5149. 

Kadrmas, J. L., Allaway, D., Studholme, R. E., Sullivan, J. T., Ronson, C. W., Poole, P. R., & Raetz, C. R. (1998). Cloning and overexpression of glycosyltransferases that generate the lipopolysaccharide core of Rhizobium leguminosarum. Journal of Biological Chemistry273(41), 26432-26440. 

Sullivan, J. T., Eardly, B. D., van Berkum, P., & Ronson, C. W. (1996). Four unnamed species of nonsymbiotic rhizobia isolated from the rhizosphere of Lotus corniculatus. Applied & Environmental Microbiology62, 2818-2825. 

Mercer, A. A., Green, G., Sullivan, J. T., Robinson, A. J., & Drillien, R. (1996). Location, DNA sequence and transcriptional analysis of the DNA polymerase gene of orf virus. Journal of General Virology77, 1563-1568. 

Mercer, A. A., Lyttle, D. J., Whelan, E., Fleming, S. B., & Sullivan, J. T. (1995). The establishment of a genetic map of orf virus reveals a pattern of genomic organisation that is highly conserved among divergent poxviruses. Virology212, 698-704. 

Fleming, S. B., Lyttle, D. J., Sullivan, J. T., Mercer, A. A., & Robinson, A. J. (1995). Genomic analysis of transposition-deletion variant of orf virus reveals a 3.3 kbp region of non-essential DNA. Journal of General Virology76, 2969-2978. 

Sullivan, J. T., Fleming, S. B., Robinson, A. J., & Mercer, A. A. (1995). Sequence and transcriptional analysis of a near-terminal region of the orf virus genome. Virus Genes11, 21-29.

Chapter in Book

Ronson, C. W., Hynes, M. F., Ramsay, J. P., & Sullivan, J. T. (2013). Symbiosis islands. In S. Maloy & K. Hughes (Eds.), Brenner's encyclopedia of genetics. (2nd ed.) (pp. 598-600). London, UK: Elsevier.  doi: 10.1016/B978-0-12-374984-0.01497-2

Ronson, C. W., Sullivan, J. T., Wijkstra, G. S., Carlton, T. M., Muirhead, K., Trzebiatowski, J. R., … de Bruijn, F. J. (2002). Genome diversity at the PHE-tRNA locus in a field population of mesorhizobia. In T. Finan, M. O'Brian, D. Layzell, J. Vessey & W. Newton (Eds.), Nitrogen Fixation: Global Perspectives. (pp. 66-74). New York and Oxford: CABI Publishing. 

de Bruijn, F. J., Ampe, F., Batut, J., Berges, H., Davalos, M., Davey, M., … Ronson, C. W., … Sullivan, J. T., … Vriezen, C. (2002). Genetics and genomics of stress - Induced gene expression in rhizobia. In T. Finan, M. O'Brian, D. Layzell, K. Vessey & W. Newton (Eds.), Nitrogen Fixation: Global Perspectives. (pp. 75-79). Oxford and New York: CABI Publishing. 

Ronson, C. W., Sullivan, J. T., Trzebiatowski, J. R., Gouzy, J., & de Bruijn, F. J. (2002). Comparative genomics of the symbiosis islands of Mesorhizobium loti. In S. A. Leong, C. Allen & E. W. Triplett (Eds.), Biology of Plant-Microbe Interactions: Volume 3. (pp. 325-330). St Paul, Minnesota, USA: International Society for Molecular Plant Microbes Interactions. 

Ronson, C. W., & Sullivan, J. T. (2001). Symbiosis islands. In S. Brenner & J. H. Miller (Eds.), Encyclopedia of Genetics. (pp. 1902-1903). London: Academic Press. 

Sullivan, J. T., Trzebiatowski, J. R., de Bruijn, F. J., & Ronson, C. W. (2000). The symbiosis island of a Mesorhizobium strain that nodulates Lotus. In E. W. Triplett (Ed.), Prokaryotic Nitrogen Fixation: A Model System for Analysis of a Biological Process. (pp. 693-704). Wymondham, UK: Horizon Scientific Press.