12.00pm Monday, 19 February
2nd floor, Microbiology Building
720 Cumberland St
Professor Martin J. Loessner
Institute of Food, Nutrition and Health
The host specificity and antimicrobial activity of bacteriophages are the basis for various diagnostic and antimicrobial/therapeutic applications, in food safety and medical settings. However, use of phage is often restricted by too narrow host ranges, transfer of potentially undesired genetic determinants, and occurrence of resistance. Thus, genome editing is required to unleash the full potential of phage therapy and biotechnology. Towards this aim, we have developed several new approaches, including in vitro assembly of full length linear or circular DNA phage genomes, employing Listeria monocytogenes cell wall-deficient L-form cells as rebooting compartments for synthetic viruses. Moreover, we identified the first functional type II CRISPR cas9 system in Listeria, and used it to develop a molecular toolbox for rapid and efficient editing of very large, non-integrating phage genomes.
This seminar is kindly sponsored by: BD Biosciences