12:00 noon Monday, 8 April
Room 208, 2nd floor
720 Cumberland St
Professor Zhi Ping (Gordon) Xu
Australian Institute for Bioengineering and Nanotechnology, University of Queensland
Recently, clay nanoparticles, i.e. layered double hydroxides (LDHs) and hectorite (HEC), appear as effective adjuvants with desirable physicochemical properties. We investigated the vaccine formulations containing the essential infectious factor IB for enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) responsible for a series of human diseases worldwide. Both LDH and HEC nanoparticles are able to carry an appreciable amount of IB, and significantly facilitate the cellular internalization of IB. The medium size LDH (115 nm) and HEC (77 nm) both induced much stronger antibody and cell-mediated immune responses than those by commercial adjuvants, QuilA, and maintained much higher levels of specific antibody immune response for at least four months. Such immune response profiles in mice show a very strong immunity for the host cells against the target bacteria in the in vitro assays. The possible mechanism for the immunity promotion using clay nanoparticles as the adjuvant be relevant to the suitable structure of formed depots.