Skip to Navigation Skip to Content Skip to Footer

Microbiology Logo Microbiology & Immunology
Te Tari Moromoroiti me te Ārai Mate

Flow Cytometry Facility


The Flow Cytometry Facility is available for studies on: multi-colour analysis, viability, cell cycle analysis, fluorescent proteins, flow FISH, apoptosis, signal transduction pathways (phosphorylation), autophagy, oncosis, necrosis, functional analysis, rare event analysis, translocation, FRET, bacteria, CBA, insects, neurons, NADPH, DNA and RNA content, transcription factors and much more!flow cytometry

All users must register and undertake basic training online and in person

- contact Michelle Wilson (North Campus) or Katie Young (South Campus)


Click on the machines below to make a booking.  

Please note there is a new booking system CLICK HERE for an overview.

The charging model has changed from 1 January 2018 - there is a single user charge for Division of Health Sciences users. Users outside of the Division are charged at 4 x the Health Sciences Rate.  Minimum booking time of 30 minutes for all resources.

FACS analysers   

BD Fortessa (MicrobiologyBeckman Coulter Gallios (Pathology)FACS Canto II (Microbiology)

$60 per hour

Cell Sorting

BD FACS Aria (Microbiology)

$120 per hour assisted sorts

$60 per hour unassisted sorts (only available for trained and approved individuals)

FACS analysis

Analysis Computer and Software (FlowJo, Spice) $10 per hour (Microbiology Room 414)

Bioplex

Bioplex Multiplex Analyser (Microbiology) $50 per hour

Technical Consultation

Assistance with experimental design or analysis.

FCF technical consultation:

Katie Young (South Campus) / Michelle Wilson (North Campus) $60 per hour

Minimum booking time 30 minutes for all resources

Training

Training will be available in March 2018 - this will be specialist workshops/seminars ($30 per person) and wet-lab workshops ($100 per person).

Jo Roberts (Flow Joanna) will also be available for one-on-one meetings ($60 per half hour).


Flow Cytometry Resources

New Users

New Users should complete this online training.

Basic Flow cytometry

Current Protocols in Cytometry (Wiley) has protocols for a wide range of flow cytometry applications (cell division, cell cycle analysis etc).

Before designing your experiment read this article on Flow Cytometry Controls, Insturment Setup and Determination of Positivity.

Multiparameter flow cytometry (>4 parameters)

Have a look at this webinar series from BD on multicolour experiments

Download a guide for designing a multicolour experiment here and a list of the Fortessa's lasers, mirrors and filters here.

To design your own panels visit Fluorofinder (which has our University of Otago machines and lasers/detectors added) or to search reagents from all major suppliers and share information visit Fluorish.

Download a guide for Principal Investigators here.

Trouble-shooting 

Subscribe to the Purdue flow cytometry group list where you can search for answers in the archive or ask questions.

Email Kelly Lundsten - BioLegend Business Segment Manager, Advanced Cytometry - for troubleshooting and multicolour panel design help

Contact FlowJoanna joanna.roberts@gmail.com for a quote for assistance with experimental design, analysis and troubleshooting.

Data analysis and presentation

The minimum information required for flow cytometry data presentation can be found here

For cloud-based data organising, analysis and visualisation visit Cytobank.

Flow Jo analysis

For data analysis visit visit the FlowJo website, where online data analysis tutorials are available.

For help with FlowJo, contact flowjo@treestar.com or jack@treestar.com

Flow cytometry societies

International Society for the Advancement of Cytometry (ISAC)

Australasian Flow Cytometry Group 

Upcoming flow cytometry meetings

Cyto2018, April 28- May 2, Prague 

Bowdoin College Annual Course in Flow Cytometry, June 16-22, Brunswick, Maine

Australasian Cytometry Society, October 21-24, 2018, Adelaide

Online webinars on a variety of topics are run throughout the year by Expert Cytometry (ExCyte)

Cyto University runs online courses throughout the year

Upcoming flow cytometry training

Training for new and advanced users will be available in March 2018 (dates to be advised). 

Special workshops/ training seminars $30 per person

Wet-lab workshops $100 per person

One-on-one meetings with flow specialist $60 per half hour


The Otago Flow Cytometry Facility Scientific Advisory Committee