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Flow Cytometry Facility


flow cytometry

The Flow Cytometry Facility is available for studies on: 

Multi-colour analysis, viability, cell cycle analysis, fluorescent proteins, flow FISH, apoptosis, signal transduction pathways (phosphorylation), autophagy, oncosis, necrosis, functional analysis, rare event analysis, translocation, FRET, bacteria, CBA, insects, neurons, NADPH, DNA and RNA content, transcription factors and much more!

All users must have registered (click here to begin) and undertaken basic training - contact Michelle Wilson, who will direct you to instrument-specific training as required.  

 Click on the machines below to make a booking.  


Multi-colour Flow Cytometry 

BD Fortessa (Microbiology)

$108 per hour ($36 PI / $72 cost centre)*

Beckman Coulter Gallios (Pathology)

$108 per hour ($36 PI / $72 cost centre)*

FACS Canto II (Microbiology)

$108 per hour ($36 PI / $72 cost centre)*

Cell Sorting

BD FACS Aria (Microbiology)

$140  per hour of sorting ($47 PI / $93cost centre) normally 2-3 hours including setup. This charge will increase to $270 per hour of sorting ($90 PI/ $180 cost centre) from 1 April 2017 to reflect actual costs.

Analysis workstation

Apple Mac Pro with analysis software available (SPICE, FlowJo 9 and 10) No charge (Microbiology Room 414)

Multiplex Array

Bioplex Multiplex Analyser (Microbiology) $50 per hour

AutoMACS Magnetic Cell Sorter 

(Microbiology) Charges vary - contact Michelle Wilson

 


Flow Cytometry Resources

Before you start:

Basic Flow cytometry

Current Protocols in Cytometry (Wiley) has protocols for a wide range of flow cytometry applications (cell division, cell cycle analysis etc).

Before designing your experiment read this article on Flow Cytometry Controls, Insturment Setup and Determination of Positivity.

Multiparameter flow cytometry (>4 parameters)

Have a look at this webinar series from BD on multicolour experiments

Download a guide for designing a multicolour experiment here and a list of the Fortessa's lasers, mirrors and filters here.

To design your own panels visit Fluorofinder (which has our University of Otago machines and lasers/detectors added) or to search reagents from all major suppliers and share information visit Fluorish.

Download a guide for Principal Investigators here.

Trouble-shooting 

Subscribe to the Purdue flow cytometry group list where you can search for answers in the archive or ask questions.

Email Kelly Lundsten - BioLegend Business Segment Manager, Advanced Cytometry - for troubleshooting and multicolour panel design help

Contact FlowJoanna joanna.roberts@gmail.com for a quote for assistance with experimental design, analysis and troubleshooting.

Data analysis and presentation

The minimum information required for flow cytometry data presentation can be found here

For cloud-based data organising, analysis and visualisation visit Cytobank.

Flow Jo analysis

For data analysis visit visit the FlowJo website, where online data analysis tutorials are available.

For help with FlowJo, contact flowjo@treestar.com or jack@treestar.com

Flow cytometry societies

International Society for the Advancement of Cytometry (ISAC)

Australasian Flow Cytometry Group 

Upcoming flow cytometry meetings

CYTO Asia 2017 October 24-27, Singapore - a combined meeting of the Australasian Cytometry Society, International Society for the Advancement of Cytometry and Singaporean Society for Immunology 

The 40th Annual Research Course in Flow Cytometry will be held at the University of New Mexico June 24-June 30th - a hands-on training course for users who already have some flow cytometry experience

Online webinars on a variety of topics are run throughout the year by Expert Cytometry (ExCyte)

Upcoming flow cytometry training

2017 training dates for new and advanced users to be advised

 

 


The Otago Flow Cytometry Facility Management Committee