The Flow Cytometry Facility is available for studies on: multi-colour analysis, viability, cell cycle analysis, fluorescent proteins, flow FISH, apoptosis, signal transduction pathways (phosphorylation), autophagy, oncosis, necrosis, functional analysis, rare event analysis, translocation, FRET, bacteria, CBA, insects, neurons, NADPH, DNA and RNA content, transcription factors and much more!
All users must register and undertake basic training online and in person
The charging model has changed from 1 January 2018 - there is a single user charge for Division of Health Sciences users. Users outside of the Division are charged at 4 x the Health Sciences Rate. Minimum booking time of 30 minutes for all resources.
$60 per hour
$120 per hour assisted sorts
$60 per hour unassisted sorts (only available for trained and approved individuals)
Analysis Computer and Software (FlowJo, Spice) $10 per hour (Microbiology Room 414)
Bioplex Multiplex Analyser (Microbiology) $50 per hour
Assistance with experimental design or analysis.
FCF technical consultation:
Katie Young (South Campus) / Michelle Wilson (North Campus) $60 per hour
Minimum booking time 30 minutes for all resources
Training will be available in March 2018 - this will be specialist workshops/seminars ($30 per person) and wet-lab workshops ($100 per person).
Jo Roberts (Flow Joanna) will also be available for one-on-one meetings ($60 per half hour).
New Users should complete this online training.
Current Protocols in Cytometry (Wiley) has protocols for a wide range of flow cytometry applications (cell division, cell cycle analysis etc).
Before designing your experiment read this article on Flow Cytometry Controls, Insturment Setup and Determination of Positivity.
Have a look at this webinar series from BD on multicolour experiments
To design your own panels visit Fluorofinder (which has our University of Otago machines and lasers/detectors added) or to search reagents from all major suppliers and share information visit Fluorish.
Download a guide for Principal Investigators here.
Subscribe to the Purdue flow cytometry group list where you can search for answers in the archive or ask questions.
Email Kelly Lundsten - BioLegend Business Segment Manager, Advanced Cytometry - for troubleshooting and multicolour panel design help
Contact FlowJoanna email@example.com for a quote for assistance with experimental design, analysis and troubleshooting.
The minimum information required for flow cytometry data presentation can be found here.
For cloud-based data organising, analysis and visualisation visit Cytobank.
For data analysis visit visit the FlowJo website, where online data analysis tutorials are available.
Cyto2018, April 28- May 2, Prague
Bowdoin College Annual Course in Flow Cytometry, June 16-22, Brunswick, Maine
Australasian Cytometry Society, October 21-24, 2018, Adelaide
Online webinars on a variety of topics are run throughout the year by Expert Cytometry (ExCyte)
Cyto University runs online courses throughout the year
Training for new and advanced users will be available in March 2018 (dates to be advised).
Special workshops/ training seminars $30 per person
Wet-lab workshops $100 per person
One-on-one meetings with flow specialist $60 per half hour