Skip to Navigation Skip to Content Skip to Footer

Microbiology Logo Microbiology & Immunology
Te Tari Moromoroiti me te Ārai Mate

Flow Cytometry Facility


The Flow Cytometry Facility is available for studies on: multi-colour analysis, viability, cell cycle analysis, fluorescent proteins, flow FISH, apoptosis, signal transduction pathways (phosphorylation), autophagy, oncosis, necrosis, functional analysis, rare event analysis, translocation, FRET, bacteria, CBA, insects, neurons, NADPH, DNA and RNA content, transcription factors and much more!flow cytometry

All users must register and undertake basic training online and in person

- contact Michelle Wilson (North Campus) or Katie Young (South Campus)


Click on the machines below to make a booking.  

Please note there is a new booking system CLICK HERE for an overview 

Multi-colour Flow Cytometry  

BD Fortessa (MicrobiologyBeckman Coulter Gallios (Pathology)FACS Canto II (Microbiology)

$108 per hour ($36 PI / $72 cost centre)*

Cell Sorting

BD FACS Aria (Microbiology)

$270 per hour of sorting ($90 PI/ $180 cost centre)

Analysis workstation

Apple Mac Pro with analysis software available (SPICE, FlowJo 9 and 10) No charge (Microbiology Room 414)

Multiplex Array

Bioplex Multiplex Analyser (Microbiology) $50 per hour

AutoMACS Magnetic Cell Sorter 

(Microbiology) Charges vary - contact Michelle Wilson 


Flow Cytometry Resources

New Users

New Users should complete this online training.

Basic Flow cytometry

Current Protocols in Cytometry (Wiley) has protocols for a wide range of flow cytometry applications (cell division, cell cycle analysis etc).

Before designing your experiment read this article on Flow Cytometry Controls, Insturment Setup and Determination of Positivity.

Multiparameter flow cytometry (>4 parameters)

Have a look at this webinar series from BD on multicolour experiments

Download a guide for designing a multicolour experiment here and a list of the Fortessa's lasers, mirrors and filters here.

To design your own panels visit Fluorofinder (which has our University of Otago machines and lasers/detectors added) or to search reagents from all major suppliers and share information visit Fluorish.

Download a guide for Principal Investigators here.

Trouble-shooting 

Subscribe to the Purdue flow cytometry group list where you can search for answers in the archive or ask questions.

Email Kelly Lundsten - BioLegend Business Segment Manager, Advanced Cytometry - for troubleshooting and multicolour panel design help

Contact FlowJoanna joanna.roberts@gmail.com for a quote for assistance with experimental design, analysis and troubleshooting.

Data analysis and presentation

The minimum information required for flow cytometry data presentation can be found here

For cloud-based data organising, analysis and visualisation visit Cytobank.

Flow Jo analysis

For data analysis visit visit the FlowJo website, where online data analysis tutorials are available.

For help with FlowJo, contact flowjo@treestar.com or jack@treestar.com

Flow cytometry societies

International Society for the Advancement of Cytometry (ISAC)

Australasian Flow Cytometry Group 

Upcoming flow cytometry meetings

CYTO Asia 2017 October 24-27, Singapore - a combined meeting of the Australasian Cytometry Society, International Society for the Advancement of Cytometry and Singaporean Society for Immunology 

Online webinars on a variety of topics are run throughout the year by Expert Cytometry (ExCyte)

Upcoming flow cytometry training

To be advised 


The Otago Flow Cytometry Facility Management Committee