The Flow Cytometry Facility is available for studies on: multi-colour analysis, viability, cell cycle analysis, fluorescent proteins, flow FISH, apoptosis, signal transduction pathways (phosphorylation), autophagy, oncosis, necrosis, functional analysis, rare event analysis, translocation, FRET, bacteria, CBA, insects, neurons, NADPH, DNA and RNA content, transcription factors and much more!
Please note there is a new booking system CLICK HERE for an overview
$108 per hour ($36 PI / $72 cost centre)*
$270 per hour of sorting ($90 PI/ $180 cost centre)
Apple Mac Pro with analysis software available (SPICE, FlowJo 9 and 10) No charge (Microbiology Room 414)
Bioplex Multiplex Analyser (Microbiology) $50 per hour
(Microbiology) Charges vary - contact Michelle Wilson
New Users should complete this online training.
Current Protocols in Cytometry (Wiley) has protocols for a wide range of flow cytometry applications (cell division, cell cycle analysis etc).
Before designing your experiment read this article on Flow Cytometry Controls, Insturment Setup and Determination of Positivity.
Have a look at this webinar series from BD on multicolour experiments
To design your own panels visit Fluorofinder (which has our University of Otago machines and lasers/detectors added) or to search reagents from all major suppliers and share information visit Fluorish.
Download a guide for Principal Investigators here.
Subscribe to the Purdue flow cytometry group list where you can search for answers in the archive or ask questions.
Email Kelly Lundsten - BioLegend Business Segment Manager, Advanced Cytometry - for troubleshooting and multicolour panel design help
Contact FlowJoanna email@example.com for a quote for assistance with experimental design, analysis and troubleshooting.
The minimum information required for flow cytometry data presentation can be found here.
For cloud-based data organising, analysis and visualisation visit Cytobank.
For data analysis visit visit the FlowJo website, where online data analysis tutorials are available.
CYTO Asia 2017 October 24-27, Singapore - a combined meeting of the Australasian Cytometry Society, International Society for the Advancement of Cytometry and Singaporean Society for Immunology
Online webinars on a variety of topics are run throughout the year by Expert Cytometry (ExCyte)
To be advised