MICR 336: Applied and Environmental Microbiology
18 points - Semester 2
Microbial technologies, including production and purification of recombinant proteins in microbes, immune technologies, bioremediation and microbial biodegradation networks. Commercialisation of biotechnology products.
Most microbes are not pathogens, rather their diversity has been exploited for a range of beneficial purposes with much of modern biotechnology studying and exploiting the ability of microbes to grow rapidly and to high levels in fermenters, to be manipulated genetically for the production of recombinant proteins, to be used in the environment for practices such as bioremediation and as technological stepping stones in discovery research.
This course in Microbial Biotechnology focuses upon the growth, manipulation and use of microbes for beneficial purposes. In particular you will:
- develop your own commercialisable biotechnology idea.
- investigate the use of recombinant bacteria and viruses for discovery, production of, and use as biotechnology products.
- be aware of the molecular systems and technologies associated with the use of microbial metabolic pathways for bioremediation.
This course is for students interested in the use of molecular microbiology for the production beneficial products and applications. It ranges from large-scale fermentation, to recombinant protein expression and purification, to concepts underlying the commercialisation of biotechnology.
Lecture course overview
The MICR 336 lecture series typically includes lectures on:
- The commercialisation of biotechnology
- Bioreactors for microbial fermentation
- Recombinant proteins and protein complexes from microorganisms
- Student team presentations on a biotechnology idea
- The applications of bacteriophage biotechnology
- Bioremediation and biosensors
Lab course overview
The MICR 336 lab classes will expose you to the use of molecular biology in biotechnology. In particular, during the 4-week lab course you will set up a gene for protein expression, express the protein in Escherichia coli and then purify the protein using a recombinant peptide tag built into the construct during cloning. You will also design your own process to isolate, express and purify a recombinant protein.
- A team case study report and oral presentation (25%)
- Lab assessment (10%)
- Final written exam (65%)
MICR 221 or MICR 201 and one further 200-level MICR, BIOC or GENE paper
Monday, Wednesday 11:00 – 11:50 am
Tuesday 14:00 – 17:50, Wednesday 09:00 – 17:50*
*you can leave the Wednesday lab session to attend other courses as needed.
Microbial Biotechnology labs run weeks 5 – 8 of Semester 2.
There will be also be small amounts of work required on additional days of the week but this can be worked around other class commitments.
Note: there are no lecture or practical clashes between any of the 300-level MICR papers
There is no required text for this course but you will be directed to relevant scientific papers during lectures and you may like to read BR Glick and JJ Pasternak 2003 Molecular Biotechnology: Principles and applications of recombinant DNA. 3rd Edition, ASM Press, which is held in the Science Library.
- Dr Sergio Morales (Course Convenor) »
- Professor Vernon Ward »
- Professor Phil Bremer »
- Dr Robin Simmonds »
- Dr Rita Przybilski
For more information on this course, please contact the Course Convenors Vernon Ward (firstname.lastname@example.org) and Sergio Morales (email@example.com) or the 3rd year Teaching Fellow Rita Przybilski (micro300level.TF@otago.ac.nz)
To find out information on the fees and other information on this paper, click here.